Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9525 | 5' | -46.7 | NC_002532.2 | + | 2117 | 0.66 | 0.755173 |
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Target: 5'- gCUGCAUCAA--AGgUgGAACGAGCu- -3' miRNA: 3'- -GACGUAGUUguUCgAgUUUGUUCGcc -5' |
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| 9525 | 5' | -46.7 | NC_002532.2 | + | 3101 | 0.67 | 0.742781 |
|
Target: 5'- -aGCAcCAGCGAGaCUgCAAGCAAGUa- -3' miRNA: 3'- gaCGUaGUUGUUC-GA-GUUUGUUCGcc -5' |
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| 9525 | 5' | -46.7 | NC_002532.2 | + | 12318 | 0.67 | 0.742781 |
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Target: 5'- -cGcCAUCGACAAGCUUGuuaccAACu-GCGGu -3' miRNA: 3'- gaC-GUAGUUGUUCGAGU-----UUGuuCGCC- -5' |
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| 9525 | 5' | -46.7 | NC_002532.2 | + | 846 | 0.67 | 0.717536 |
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Target: 5'- -gGCAUCAauGCGAGCUCuggaGGGCu- -3' miRNA: 3'- gaCGUAGU--UGUUCGAGuuugUUCGcc -5' |
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| 9525 | 5' | -46.7 | NC_002532.2 | + | 7060 | 0.67 | 0.704723 |
|
Target: 5'- -gGCu---GCcGGUUCAAGCAAGUGGa -3' miRNA: 3'- gaCGuaguUGuUCGAGUUUGUUCGCC- -5' |
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| 9525 | 5' | -46.7 | NC_002532.2 | + | 11725 | 0.68 | 0.664446 |
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Target: 5'- -gGUAUCGcCAAGC-CAGGCAaaccacaguaauuGGCGGa -3' miRNA: 3'- gaCGUAGUuGUUCGaGUUUGU-------------UCGCC- -5' |
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| 9525 | 5' | -46.7 | NC_002532.2 | + | 7898 | 1.14 | 0.000488 |
|
Target: 5'- aCUGCAUCAACAAGCUCAAACAAGCGGg -3' miRNA: 3'- -GACGUAGUUGUUCGAGUUUGUUCGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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