miRNA: 3'- -GUU--UUACCUUGUGg--GU-----UUGCGAUGau -5'
miRNA: 3'- guuuuACCUu---GUGG--GUUUGCGAUGAU- -5'
miRNA: 3'- guUUUACCuUGUGGGUUU--GC-GAUGAU- -5'
miRNA: 3'- guUUUACCuu------------GUGGGUUUGCGAUGau -5'
miRNA: 3'- -GUUUUACCUUGUGGGUUUGCGAUGAU- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9527 | 3' | -45.7 | NC_002532.2 | + | 9288 | 0.67 | 0.763916 |
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Target: 5'- uCAAauGAUGGAGCAUagaCAucaacAACGCUGCa- -3' miRNA: 3'- -GUU--UUACCUUGUGg--GU-----UUGCGAUGau -5' |
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| 9527 | 3' | -45.7 | NC_002532.2 | + | 6979 | 0.67 | 0.738781 |
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Target: 5'- ----cUGGGucccCACCugCAGGCGCUGCUAg -3' miRNA: 3'- guuuuACCUu---GUGG--GUUUGCGAUGAU- -5' |
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| 9527 | 3' | -45.7 | NC_002532.2 | + | 4937 | 0.68 | 0.686774 |
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Target: 5'- -cAAAUGGuGCACCUAGAacCGcCUACUAc -3' miRNA: 3'- guUUUACCuUGUGGGUUU--GC-GAUGAU- -5' |
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| 9527 | 3' | -45.7 | NC_002532.2 | + | 7601 | 0.7 | 0.594828 |
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Target: 5'- -cGGAUGGucgaacaacaucacCGCCCGGGCGCUACa- -3' miRNA: 3'- guUUUACCuu------------GUGGGUUUGCGAUGau -5' |
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| 9527 | 3' | -45.7 | NC_002532.2 | + | 10940 | 1.08 | 0.001617 |
|
Target: 5'- cCAAAAUGGAACACCCAAACGCUACUAu -3' miRNA: 3'- -GUUUUACCUUGUGGGUUUGCGAUGAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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