Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9915 | 3' | -58.4 | NC_002641.1 | + | 154147 | 0.66 | 0.815514 |
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Target: 5'- aCGCcuuuugGGGGCAGUUUCcacgggaUCgCUGGCGGa -3' miRNA: 3'- aGCG------CCCCGUUAAAG-------AGgGGCCGCUg -5' |
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| 9915 | 3' | -58.4 | NC_002641.1 | + | 128326 | 0.66 | 0.815514 |
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Target: 5'- aCGCcuuuugGGGGCAGUUUCcacgggaUCgCUGGCGGa -3' miRNA: 3'- aGCG------CCCCGUUAAAG-------AGgGGCCGCUg -5' |
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| 9915 | 3' | -58.4 | NC_002641.1 | + | 116619 | 0.67 | 0.790204 |
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Target: 5'- aCGCGGac--GUUUC-CCCCGGUGGCc -3' miRNA: 3'- aGCGCCccguUAAAGaGGGGCCGCUG- -5' |
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| 9915 | 3' | -58.4 | NC_002641.1 | + | 124133 | 0.68 | 0.734419 |
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Target: 5'- -aGCGGGGC---UUCUCUUugggagagUGGCGACu -3' miRNA: 3'- agCGCCCCGuuaAAGAGGG--------GCCGCUG- -5' |
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| 9915 | 3' | -58.4 | NC_002641.1 | + | 158340 | 0.68 | 0.734419 |
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Target: 5'- -aGCGGGGC---UUCUCUUugggagagUGGCGACu -3' miRNA: 3'- agCGCCCCGuuaAAGAGGG--------GCCGCUG- -5' |
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| 9915 | 3' | -58.4 | NC_002641.1 | + | 146577 | 0.69 | 0.685504 |
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Target: 5'- uUUGCGucGaCAAUUUacaCUUCCCGGCGACg -3' miRNA: 3'- -AGCGCccC-GUUAAA---GAGGGGCCGCUG- -5' |
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| 9915 | 3' | -58.4 | NC_002641.1 | + | 135895 | 0.69 | 0.685504 |
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Target: 5'- uUUGCGucGaCAAUUUacaCUUCCCGGCGACg -3' miRNA: 3'- -AGCGCccC-GUUAAA---GAGGGGCCGCUG- -5' |
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| 9915 | 3' | -58.4 | NC_002641.1 | + | 154465 | 1.09 | 0.0019 |
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Target: 5'- aUCGCGGGGCAAUUUCUCCCCGGCGACc -3' miRNA: 3'- -AGCGCCCCGUUAAAGAGGGGCCGCUG- -5' |
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| 9915 | 3' | -58.4 | NC_002641.1 | + | 128007 | 1.09 | 0.0019 |
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Target: 5'- aUCGCGGGGCAAUUUCUCCCCGGCGACc -3' miRNA: 3'- -AGCGCCCCGUUAAAGAGGGGCCGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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