Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9915 | 5' | -50.6 | NC_002641.1 | + | 53901 | 0.66 | 0.991352 |
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Target: 5'- ---aUGUGGGCCAGUgauuuugACAGaaugGGCUGg -3' miRNA: 3'- uauaGCGCCUGGUCA-------UGUUaa--CCGAC- -5' |
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| 9915 | 5' | -50.6 | NC_002641.1 | + | 15873 | 0.68 | 0.976979 |
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Target: 5'- -aGUCGCGuccauCCGGgaaGAUUGGCUGg -3' miRNA: 3'- uaUAGCGCcu---GGUCaugUUAACCGAC- -5' |
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| 9915 | 5' | -50.6 | NC_002641.1 | + | 66055 | 0.68 | 0.968408 |
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Target: 5'- --uUCGCGGACCAGUGuCAAca-GCUc -3' miRNA: 3'- uauAGCGCCUGGUCAU-GUUaacCGAc -5' |
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| 9915 | 5' | -50.6 | NC_002641.1 | + | 5565 | 0.73 | 0.837444 |
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Target: 5'- ---cCGCGGACCGGU-CAAUgUGGCg- -3' miRNA: 3'- uauaGCGCCUGGUCAuGUUA-ACCGac -5' |
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| 9915 | 5' | -50.6 | NC_002641.1 | + | 118157 | 0.73 | 0.837444 |
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Target: 5'- ---cCGCGGACCGGU-CAAUgUGGCg- -3' miRNA: 3'- uauaGCGCCUGGUCAuGUUA-ACCGac -5' |
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| 9915 | 5' | -50.6 | NC_002641.1 | + | 154431 | 1.06 | 0.012577 |
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Target: 5'- gAUAUCGCGGACCAGUACAAUUGGCUGc -3' miRNA: 3'- -UAUAGCGCCUGGUCAUGUUAACCGAC- -5' |
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| 9915 | 5' | -50.6 | NC_002641.1 | + | 128041 | 1.06 | 0.012577 |
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Target: 5'- gAUAUCGCGGACCAGUACAAUUGGCUGc -3' miRNA: 3'- -UAUAGCGCCUGGUCAUGUUAACCGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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