Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9917 | 5' | -51.7 | NC_002641.1 | + | 147611 | 0.68 | 0.954378 |
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Target: 5'- aACAGCCGUUguggaaugCGUGCAuuccuugcauuGCGCUGa-- -3' miRNA: 3'- -UGUCGGCAAaa------GCAUGU-----------CGCGGCaac -5' |
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| 9917 | 5' | -51.7 | NC_002641.1 | + | 134861 | 0.68 | 0.954378 |
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Target: 5'- aACAGCCGUUguggaaugCGUGCAuuccuugcauuGCGCUGa-- -3' miRNA: 3'- -UGUCGGCAAaa------GCAUGU-----------CGCGGCaac -5' |
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| 9917 | 5' | -51.7 | NC_002641.1 | + | 145902 | 0.69 | 0.941165 |
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Target: 5'- gGCAGCCGUcg--GUAC-GCGCCGa-- -3' miRNA: 3'- -UGUCGGCAaaagCAUGuCGCGGCaac -5' |
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| 9917 | 5' | -51.7 | NC_002641.1 | + | 136571 | 0.69 | 0.941165 |
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Target: 5'- gGCAGCCGUcg--GUAC-GCGCCGa-- -3' miRNA: 3'- -UGUCGGCAaaagCAUGuCGCGGCaac -5' |
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| 9917 | 5' | -51.7 | NC_002641.1 | + | 142911 | 0.72 | 0.833213 |
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Target: 5'- uACAGCUGgUUUU-UGCAGCGCCGUc- -3' miRNA: 3'- -UGUCGGCaAAAGcAUGUCGCGGCAac -5' |
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| 9917 | 5' | -51.7 | NC_002641.1 | + | 157402 | 1.08 | 0.00914 |
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Target: 5'- cACAGCCGUUUUCGUACAGCGCCGUUGc -3' miRNA: 3'- -UGUCGGCAAAAGCAUGUCGCGGCAAC- -5' |
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| 9917 | 5' | -51.7 | NC_002641.1 | + | 125070 | 1.08 | 0.00914 |
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Target: 5'- cACAGCCGUUUUCGUACAGCGCCGUUGc -3' miRNA: 3'- -UGUCGGCAAAAGCAUGUCGCGGCAAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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