Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9983 | 3' | -42.4 | NC_002645.1 | + | 13661 | 0.66 | 0.996656 |
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Target: 5'- -----cGCUGCUuUGAgUACAGGAUu -3' miRNA: 3'- aauaauCGAUGAuACUgAUGUUCUGu -5' |
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| 9983 | 3' | -42.4 | NC_002645.1 | + | 14238 | 0.66 | 0.996656 |
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Target: 5'- ---gUAGCUACUAUGGauuucAGACAu -3' miRNA: 3'- aauaAUCGAUGAUACUgauguUCUGU- -5' |
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| 9983 | 3' | -42.4 | NC_002645.1 | + | 8295 | 0.68 | 0.984133 |
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Target: 5'- -cAUUAGCUugUAuUGAaCUGUAAGGCAa -3' miRNA: 3'- aaUAAUCGAugAU-ACU-GAUGUUCUGU- -5' |
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| 9983 | 3' | -42.4 | NC_002645.1 | + | 24395 | 0.69 | 0.975899 |
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Target: 5'- --cUUGGC-ACUGcUGGCUcuACAAGACAu -3' miRNA: 3'- aauAAUCGaUGAU-ACUGA--UGUUCUGU- -5' |
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| 9983 | 3' | -42.4 | NC_002645.1 | + | 3944 | 0.69 | 0.964904 |
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Target: 5'- aUGUUAGuCUGgUaAUGACcACAAGACAc -3' miRNA: 3'- aAUAAUC-GAUgA-UACUGaUGUUCUGU- -5' |
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| 9983 | 3' | -42.4 | NC_002645.1 | + | 14149 | 1.08 | 0.00928 |
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Target: 5'- uUUAUUAGCUACUAUGACUACAAGACAg -3' miRNA: 3'- -AAUAAUCGAUGAUACUGAUGUUCUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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