Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9988 | 5' | -46.7 | NC_002654.1 | + | 99596 | 0.66 | 0.999802 |
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Target: 5'- uUUGGCGAGGAgugCGUUcGCUGCaaaCUGAa -3' miRNA: 3'- uAACUGCUUUUa--GUAGcUGACG---GACU- -5' |
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| 9988 | 5' | -46.7 | NC_002654.1 | + | 22077 | 0.67 | 0.999328 |
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Target: 5'- -aUGGCGAAuuguacuGGUCGUCuacaacGACggcgGCCUGAu -3' miRNA: 3'- uaACUGCUU-------UUAGUAG------CUGa---CGGACU- -5' |
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| 9988 | 5' | -46.7 | NC_002654.1 | + | 129769 | 0.69 | 0.99603 |
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Target: 5'- cGUUGACGcGAGUCGUCGAaacgacagagucuuUUGCCa-- -3' miRNA: 3'- -UAACUGCuUUUAGUAGCU--------------GACGGacu -5' |
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| 9988 | 5' | -46.7 | NC_002654.1 | + | 30904 | 0.7 | 0.992865 |
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Target: 5'- cGUUGACGAAGuguuccUCGUCGAC-GCCcaUGGa -3' miRNA: 3'- -UAACUGCUUUu-----AGUAGCUGaCGG--ACU- -5' |
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| 9988 | 5' | -46.7 | NC_002654.1 | + | 80414 | 0.7 | 0.989039 |
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Target: 5'- uGUUGACGGccAAAUCGUCGACcaguguuaUGCUcuUGAu -3' miRNA: 3'- -UAACUGCU--UUUAGUAGCUG--------ACGG--ACU- -5' |
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| 9988 | 5' | -46.7 | NC_002654.1 | + | 99720 | 0.89 | 0.262602 |
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Target: 5'- cAUUGACGAAAAUCAUCGACUGCg--- -3' miRNA: 3'- -UAACUGCUUUUAGUAGCUGACGgacu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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