Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9991 | 3' | -54.9 | NC_002654.1 | + | 7741 | 0.66 | 0.9313 |
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Target: 5'- cGUCGUCUGUG-UCAUCGUUCaAACa -3' miRNA: 3'- cUAGCAGACGCuGGUGGCGAGcUUGc -5' |
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| 9991 | 3' | -54.9 | NC_002654.1 | + | 114368 | 0.66 | 0.91472 |
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Target: 5'- cGUCGUCUGUGACaagUACgGCUgCGGcuACGg -3' miRNA: 3'- cUAGCAGACGCUG---GUGgCGA-GCU--UGC- -5' |
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| 9991 | 3' | -54.9 | NC_002654.1 | + | 82308 | 0.68 | 0.867718 |
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Target: 5'- --aCGUCUaCGGCCGCCGCcgccgccUGAGCGu -3' miRNA: 3'- cuaGCAGAcGCUGGUGGCGa------GCUUGC- -5' |
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| 9991 | 3' | -54.9 | NC_002654.1 | + | 82269 | 0.68 | 0.83603 |
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Target: 5'- --gCGUC-GaCGACCGCCGCUaGAACu -3' miRNA: 3'- cuaGCAGaC-GCUGGUGGCGAgCUUGc -5' |
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| 9991 | 3' | -54.9 | NC_002654.1 | + | 82244 | 0.71 | 0.694851 |
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Target: 5'- -cUCGUCUaCcGCCACCGCUgGAACu -3' miRNA: 3'- cuAGCAGAcGcUGGUGGCGAgCUUGc -5' |
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| 9991 | 3' | -54.9 | NC_002654.1 | + | 68553 | 0.71 | 0.694851 |
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Target: 5'- aAUCGUCUGuCGAaaauuUCACCGC-CGGACa -3' miRNA: 3'- cUAGCAGAC-GCU-----GGUGGCGaGCUUGc -5' |
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| 9991 | 3' | -54.9 | NC_002654.1 | + | 127756 | 0.73 | 0.581784 |
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Target: 5'- cGAUCG-UUGCGcACCACCGUUCGugUGg -3' miRNA: 3'- -CUAGCaGACGC-UGGUGGCGAGCuuGC- -5' |
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| 9991 | 3' | -54.9 | NC_002654.1 | + | 43744 | 0.74 | 0.521303 |
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Target: 5'- -uUCGUC-GCGGCCACUGCU-GAACa -3' miRNA: 3'- cuAGCAGaCGCUGGUGGCGAgCUUGc -5' |
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| 9991 | 3' | -54.9 | NC_002654.1 | + | 82129 | 0.87 | 0.100835 |
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Target: 5'- ---nGUCUGCGACCACCGCcCGAACGc -3' miRNA: 3'- cuagCAGACGCUGGUGGCGaGCUUGC- -5' |
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| 9991 | 3' | -54.9 | NC_002654.1 | + | 82104 | 1.08 | 0.003764 |
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Target: 5'- aGAUCGUCUGCGACCACCGCUCGAACGu -3' miRNA: 3'- -CUAGCAGACGCUGGUGGCGAGCUUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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