miRNA: 3'- -UGUCGU----GCAGUGaaGGUGAUUCU- -5'
miRNA: 3'- -UGUCGUGCAgUGaAGGUGauucu -5'
miRNA: 3'- -UGUCGUGCAgUGa---AGGUGaUUCU- -5'
miRNA: 3'- -UGUCGUGCaGUGa---AGGUGAUUCU- -5'
miRNA: 3'- -UGUCGUGCAGUGAAGGUGAUUCU- -5'
Results 1 - 5 of 5 are showing below:
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| Acc. No. | Name | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| MIMAT0000999 | ebv-miR-BART1-5p | -47.2 | NC_007605.1 | + | 152359 | 0.67 | 0.99759 |
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Target: 5'- aGCAGCAggcuUGUCAUg-CCGCUGAGGa -3' miRNA: 3'- -UGUCGU----GCAGUGaaGGUGAUUCU- -5' |
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| MIMAT0000999 | ebv-miR-BART1-5p | -47.2 | NC_007605.1 | + | 155419 | 0.67 | 0.99759 |
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Target: 5'- aGCGGCGCGUgGCaUCCACg---- -3' miRNA: 3'- -UGUCGUGCAgUGaAGGUGauucu -5' |
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| MIMAT0000999 | ebv-miR-BART1-5p | -47.2 | NC_007605.1 | + | 155563 | 0.68 | 0.995158 |
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Target: 5'- aACGGCGCGUgACcuucUCCACcGAGAc -3' miRNA: 3'- -UGUCGUGCAgUGa---AGGUGaUUCU- -5' |
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| MIMAT0000999 | ebv-miR-BART1-5p | -47.2 | NC_007605.1 | - | 97755 | 0.71 | 0.96875 |
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Target: 5'- aACAGCACG-CAUgaugUCUACUGGGGa -3' miRNA: 3'- -UGUCGUGCaGUGa---AGGUGAUUCU- -5' |
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| MIMAT0000999 | ebv-miR-BART1-5p | -47.2 | NC_007605.1 | - | 139406 | 1.01 | 0.041764 |
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Target: 5'- cACAGCACGUCACUUCCACUAAGAc -3' miRNA: 3'- -UGUCGUGCAGUGAAGGUGAUUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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