miRNA: 3'- -CGuuCCCGCUUa---CGUCuuUUau -5'
miRNA: 3'- -CGUUCCCGc-UUACGUCUUUuau -5'
miRNA: 3'- -CGUUCCCG-----------CUUACGUCUUUUau -5'
miRNA: 3'- -CGUUCCCGCUuaCGUCUuuuau -5'
miRNA: 3'- -CGUUCCCGCUUACGUCUUUUAU- -5'
Results 41 - 45 of 45 are showing below:
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| Acc. No. | Name | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| MIMAT0001000 | ebv-miR-BART2 | -42.5 | NC_007605.1 | - | 62319 | 0.72 | 0.99772 |
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Target: 5'- aGUggGGGCGGGgccgGCGGggGAg- -3' miRNA: 3'- -CGuuCCCGCUUa---CGUCuuUUau -5' |
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| MIMAT0001000 | ebv-miR-BART2 | -42.5 | NC_007605.1 | + | 72288 | 0.74 | 0.99216 |
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Target: 5'- aGCGAGGGCcaGAUGCAGGAGc-- -3' miRNA: 3'- -CGUUCCCGc-UUACGUCUUUuau -5' |
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| MIMAT0001000 | ebv-miR-BART2 | -42.5 | NC_007605.1 | - | 148971 | 0.74 | 0.99216 |
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Target: 5'- cGCAGGGGCgccgaaagcagGAAUGCGGGGAGc- -3' miRNA: 3'- -CGUUCCCG-----------CUUACGUCUUUUau -5' |
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| MIMAT0001000 | ebv-miR-BART2 | -42.5 | NC_007605.1 | + | 61445 | 0.74 | 0.990849 |
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Target: 5'- gGCGGGGGCGGcgGCGGGc---- -3' miRNA: 3'- -CGUUCCCGCUuaCGUCUuuuau -5' |
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| MIMAT0001000 | ebv-miR-BART2 | -42.5 | NC_007605.1 | - | 152788 | 1.02 | 0.094377 |
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Target: 5'- cGCAAGGGCGAAUGCAGAAAAUAg -3' miRNA: 3'- -CGUUCCCGCUUACGUCUUUUAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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