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miRNA: 3'- -CUCUGCUGGG--------------CUAG-AGU--UGAGa -5'
miRNA: 3'- -CUCUGCUGGGCU-AGAGU------UGAGa -5'
miRNA: 3'- -CUCUGCUGGGCUAGAGUUGAGA- -5'
Results 1 - 3 of 3 are showing below:
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| Acc. No. | Name | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio#
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P value |
| Predicted miRNA align pattern | |||||||
| MIMAT0001568 | mghv-miR-M1-5 | -47.3 | NC_001826.1 | + | 117699 | 0.65 | 0.997493 |
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Target: 5'- aGAGGCGGCCCccucuggguggccaGAUCuUCAgcGCUCg -3' miRNA: 3'- -CUCUGCUGGG--------------CUAG-AGU--UGAGa -5' |
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| MIMAT0001568 | mghv-miR-M1-5 | -47.3 | NC_001826.1 | - | 64627 | 0.67 | 0.992215 |
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Target: 5'- aGGGACGGCCUGGuUCUCAccacaaACUUg -3' miRNA: 3'- -CUCUGCUGGGCU-AGAGU------UGAGa -5' |
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| MIMAT0001568 | mghv-miR-M1-5 | -47.3 | NC_001826.1 | - | 1396 | 1.01 | 0.030939 |
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Target: 5'- gGAGACGACCCGAUCUCAACUCUg -3' miRNA: 3'- -CUCUGCUGGGCUAGAGUUGAGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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