miRNA: 3'- gaUGUUu--------GAUCGUAAGACCAcu -5'
miRNA: 3'- -GAUGUUUGAUCGUAAGACCACU- -5'
miRNA: 3'- -GAUGUUu-GAUCGUAAGACCacu -5'
miRNA: 3'- gAUGU--UUGAUCGuaAGACC-ACU- -5'
miRNA: 3'- -GAUGUUUGa------------UCGUAAGACCACU- -5'
Results 1 - 5 of 5 are showing below:
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| Acc. No. | Name | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| MIMAT0001575 | hcmv-miR-UL22A* | -41.3 | NC_006273.1 | + | 12209 | 0.68 | 1 |
|
Target: 5'- -aACAAuuauccuauCUAGCAUUCUGGUa- -3' miRNA: 3'- gaUGUUu--------GAUCGUAAGACCAcu -5' |
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| MIMAT0001575 | hcmv-miR-UL22A* | -41.3 | NC_006273.1 | - | 28258 | 1.02 | 0.184019 |
|
Target: 5'- uCUACAAACUAGCAUUCUGGUGAg -3' miRNA: 3'- -GAUGUUUGAUCGUAAGACCACU- -5' |
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| MIMAT0001575 | hcmv-miR-UL22A* | -41.3 | NC_006273.1 | - | 66654 | 0.66 | 1 |
|
Target: 5'- uCUGCAAcuCUAGCAUUUUGGc-- -3' miRNA: 3'- -GAUGUUu-GAUCGUAAGACCacu -5' |
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| MIMAT0001575 | hcmv-miR-UL22A* | -41.3 | NC_006273.1 | - | 91957 | 0.67 | 1 |
|
Target: 5'- gUGCGucGGCUAGCggUCUGGcUGGa -3' miRNA: 3'- gAUGU--UUGAUCGuaAGACC-ACU- -5' |
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| MIMAT0001575 | hcmv-miR-UL22A* | -41.3 | NC_006273.1 | + | 208537 | 0.69 | 1 |
|
Target: 5'- gCUGCGGAUgauguggcucacgGGCAUUUUGGUGGc -3' miRNA: 3'- -GAUGUUUGa------------UCGUAAGACCACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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