miRNA: 3'- agaaaGGUCCACAGa-------AGUUGCU- -5'
miRNA: 3'- -AGaaAGGuCCACAGAAG-UugCU- -5'
miRNA: 3'- agaaaGGUCCAC-------AGAAGUUGCU- -5'
miRNA: 3'- -AGAAAGGUCCACAGAAGUUGCu -5'
miRNA: 3'- -AGAAAGGUCCACAGAAGUUGCU- -5'
Results 1 - 5 of 5 are showing below:
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| Acc. No. | Name | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| MIMAT0001576 | hcmv-miR-UL36 | -43.8 | NC_006273.1 | + | 201120 | 0.68 | 0.999988 |
|
Target: 5'- ----cCCGGGUGUCgcgacaugUCGACGAc -3' miRNA: 3'- agaaaGGUCCACAGa-------AGUUGCU- -5' |
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| MIMAT0001576 | hcmv-miR-UL36 | -43.8 | NC_006273.1 | + | 174813 | 0.69 | 0.999938 |
|
Target: 5'- aUCggUCCcGGUGUCUUCuAugGAg -3' miRNA: 3'- -AGaaAGGuCCACAGAAG-UugCU- -5' |
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| MIMAT0001576 | hcmv-miR-UL36 | -43.8 | NC_006273.1 | + | 168040 | 0.66 | 1 |
|
Target: 5'- ----aCCGGGUGuuucuuuUCUUCAACGGg -3' miRNA: 3'- agaaaGGUCCAC-------AGAAGUUGCU- -5' |
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| MIMAT0001576 | hcmv-miR-UL36 | -43.8 | NC_006273.1 | - | 50058 | 0.9 | 0.430444 |
|
Target: 5'- uUCUUUCCAGGUGUUUUCAACGu -3' miRNA: 3'- -AGAAAGGUCCACAGAAGUUGCu -5' |
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| MIMAT0001576 | hcmv-miR-UL36 | -43.8 | NC_006273.1 | + | 49843 | 1.01 | 0.123218 |
|
Target: 5'- cUCUUUCCAGGUGUCUUCAACGAc -3' miRNA: 3'- -AGAAAGGUCCACAGAAGUUGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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