miRNA display CGI

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Acc. No.	Name	Perfect MFE.*	RefSeq Acc.	strand	Start Position	R_P_ratio#	P value
	Predicted miRNA align pattern
MIMAT0001579	hcmv-miR-US5-1	-46.7	NC_006273.1	-	36353	0.72	0.978067
	Target:  5'- cGCGCUCUUGUgucucccgaCAGGCUcGUCGu -3' miRNA:   3'- -UGCGAGAGCA---------GUCCGAaCAGU- -5'
MIMAT0001579	hcmv-miR-US5-1	-46.7	NC_006273.1	-	48020	0.68	0.998
	Target:  5'- aGCGCUUgggUGUCAGGCccGUCu -3' miRNA:   3'- -UGCGAGa--GCAGUCCGaaCAGu -5'
MIMAT0001579	hcmv-miR-US5-1	-46.7	NC_006273.1	+	56186	0.7	0.995194
	Target:  5'- gACGCUgUCGUCcGGCcaGUCAa -3' miRNA:   3'- -UGCGAgAGCAGuCCGaaCAGU- -5'
MIMAT0001579	hcmv-miR-US5-1	-46.7	NC_006273.1	-	103666	0.69	0.995924
	Target:  5'- aACGCUCccUGUCcGGCUUGUUAc -3' miRNA:   3'- -UGCGAGa-GCAGuCCGAACAGU- -5'
MIMAT0001579	hcmv-miR-US5-1	-46.7	NC_006273.1	-	186355	0.68	0.998896
	Target:  5'- cGCGCUCUCaucucuUCAGaCUUGUCAu -3' miRNA:   3'- -UGCGAGAGc-----AGUCcGAACAGU- -5'
MIMAT0001579	hcmv-miR-US5-1	-46.7	NC_006273.1	-	202371	1.01	0.055769
	Target:  5'- aACGCUCUCGUCAGGCUUGUCAu -3' miRNA:   3'- -UGCGAGAGCAGUCCGAACAGU- -5'

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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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