miRNA display CGI


Results 1 - 9 of 9 are showing below:
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Acc. No. Name Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
MIMAT0001583 hcmv-miR-US25-2-3p -54.5 NC_006273.1 - 195757 0.66 0.955146
Target:  5'- gCCGCGGacuggguccccGAGCgcgcaccCUCUAGGUGGAc -3'
miRNA:   3'- -GGCGCC-----------CUCGa------GAGGUUCACCUa -5'
MIMAT0001583 hcmv-miR-US25-2-3p -54.5 NC_006273.1 + 51935 0.66 0.951068
Target:  5'- aCGUGGGAGCgcacCUCCAccgGGAa -3'
miRNA:   3'- gGCGCCCUCGa---GAGGUucaCCUa -5'
MIMAT0001583 hcmv-miR-US25-2-3p -54.5 NC_006273.1 + 167540 0.67 0.921458
Target:  5'- uCUGCGGGuGCUgaCCGAGUGcGGg -3'
miRNA:   3'- -GGCGCCCuCGAgaGGUUCAC-CUa -5'
MIMAT0001583 hcmv-miR-US25-2-3p -54.5 NC_006273.1 - 21955 0.67 0.903298
Target:  5'- gUCGCaGGAGCggUCCGAGUGGc- -3'
miRNA:   3'- -GGCGcCCUCGagAGGUUCACCua -5'
MIMAT0001583 hcmv-miR-US25-2-3p -54.5 NC_006273.1 - 39975 0.69 0.836134
Target:  5'- gCCGCGGuuGCaCUCCgAAGUGGAUa -3'
miRNA:   3'- -GGCGCCcuCGaGAGG-UUCACCUA- -5'
MIMAT0001583 hcmv-miR-US25-2-3p -54.5 NC_006273.1 + 162528 0.71 0.743624
Target:  5'- cUCGCGucacGGuGCUUUCCGAGUGGAc -3'
miRNA:   3'- -GGCGC----CCuCGAGAGGUUCACCUa -5'
MIMAT0001583 hcmv-miR-US25-2-3p -54.5 NC_006273.1 + 234327 0.71 0.713447
Target:  5'- cCCGCGGG-GUUCUaCCcGGUGGAc -3'
miRNA:   3'- -GGCGCCCuCGAGA-GGuUCACCUa -5'
MIMAT0001583 hcmv-miR-US25-2-3p -54.5 NC_006273.1 - 196212 0.71 0.713447
Target:  5'- cCCGCGGG-GUUCUaCCcGGUGGAc -3'
miRNA:   3'- -GGCGCCCuCGAGA-GGuUCACCUa -5'
MIMAT0001583 hcmv-miR-US25-2-3p -54.5 NC_006273.1 + 221457 1.02 0.011403
Target:  5'- aCCGCGGGAGCUCUCCAAGUGGAUg -3'
miRNA:   3'- -GGCGCCCUCGAGAGGUUCACCUA- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.