Results 1 - 9 of 9 are showing below:
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Acc. No. | Name | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
MIMAT0001583 | hcmv-miR-US25-2-3p | -54.5 | NC_006273.1 | + | 234327 | 0.71 | 0.713447 |
Target: 5'- cCCGCGGG-GUUCUaCCcGGUGGAc -3' miRNA: 3'- -GGCGCCCuCGAGA-GGuUCACCUa -5' |
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MIMAT0001583 | hcmv-miR-US25-2-3p | -54.5 | NC_006273.1 | + | 221457 | 1.02 | 0.011403 |
Target: 5'- aCCGCGGGAGCUCUCCAAGUGGAUg -3' miRNA: 3'- -GGCGCCCUCGAGAGGUUCACCUA- -5' |
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MIMAT0001583 | hcmv-miR-US25-2-3p | -54.5 | NC_006273.1 | - | 196212 | 0.71 | 0.713447 |
Target: 5'- cCCGCGGG-GUUCUaCCcGGUGGAc -3' miRNA: 3'- -GGCGCCCuCGAGA-GGuUCACCUa -5' |
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MIMAT0001583 | hcmv-miR-US25-2-3p | -54.5 | NC_006273.1 | - | 195757 | 0.66 | 0.955146 |
Target: 5'- gCCGCGGacuggguccccGAGCgcgcaccCUCUAGGUGGAc -3' miRNA: 3'- -GGCGCC-----------CUCGa------GAGGUUCACCUa -5' |
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MIMAT0001583 | hcmv-miR-US25-2-3p | -54.5 | NC_006273.1 | + | 167540 | 0.67 | 0.921458 |
Target: 5'- uCUGCGGGuGCUgaCCGAGUGcGGg -3' miRNA: 3'- -GGCGCCCuCGAgaGGUUCAC-CUa -5' |
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MIMAT0001583 | hcmv-miR-US25-2-3p | -54.5 | NC_006273.1 | + | 162528 | 0.71 | 0.743624 |
Target: 5'- cUCGCGucacGGuGCUUUCCGAGUGGAc -3' miRNA: 3'- -GGCGC----CCuCGAGAGGUUCACCUa -5' |
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MIMAT0001583 | hcmv-miR-US25-2-3p | -54.5 | NC_006273.1 | + | 51935 | 0.66 | 0.951068 |
Target: 5'- aCGUGGGAGCgcacCUCCAccgGGAa -3' miRNA: 3'- gGCGCCCUCGa---GAGGUucaCCUa -5' |
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MIMAT0001583 | hcmv-miR-US25-2-3p | -54.5 | NC_006273.1 | - | 39975 | 0.69 | 0.836134 |
Target: 5'- gCCGCGGuuGCaCUCCgAAGUGGAUa -3' miRNA: 3'- -GGCGCCcuCGaGAGG-UUCACCUA- -5' |
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MIMAT0001583 | hcmv-miR-US25-2-3p | -54.5 | NC_006273.1 | - | 21955 | 0.67 | 0.903298 |
Target: 5'- gUCGCaGGAGCggUCCGAGUGGc- -3' miRNA: 3'- -GGCGcCCUCGagAGGUUCACCua -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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