Results 21 - 28 of 28 are showing below:
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| Acc. No. | Name | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| MIMAT0002185 | kshv-miR-K12-9 | -46.8 | NC_003409.1 | - | 84851 | 0.69 | 0.985869 |
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Target: 5'- aUugGguGUUGUGUAUAUCCGc -3' miRNA: 3'- aAugCguCGACGCAUAUGGGUc -5' |
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| MIMAT0002185 | kshv-miR-K12-9 | -46.8 | NC_003409.1 | - | 87025 | 0.72 | 0.935339 |
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Target: 5'- ---aGCGGCgggagUGUGUGUGCCCGGg -3' miRNA: 3'- aaugCGUCG-----ACGCAUAUGGGUC- -5' |
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| MIMAT0002185 | kshv-miR-K12-9 | -46.8 | NC_003409.1 | - | 114379 | 0.67 | 0.993175 |
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Target: 5'- -aAUGCAGCacgguUGCGUGUACCa-- -3' miRNA: 3'- aaUGCGUCG-----ACGCAUAUGGguc -5' |
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| MIMAT0002185 | kshv-miR-K12-9 | -46.8 | NC_003409.1 | - | 115413 | 0.67 | 0.994184 |
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Target: 5'- --gUGCAGCUGCaagucgAUACCCAu -3' miRNA: 3'- aauGCGUCGACGca----UAUGGGUc -5' |
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| MIMAT0002185 | kshv-miR-K12-9 | -46.8 | NC_003409.1 | - | 119337 | 0.9 | 0.15678 |
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Target: 5'- aUACGCAGCUGCGUAaACCCGGc -3' miRNA: 3'- aAUGCGUCGACGCAUaUGGGUC- -5' |
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| MIMAT0002185 | kshv-miR-K12-9 | -46.8 | NC_003409.1 | - | 119393 | 0.71 | 0.955263 |
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Target: 5'- aUACcCAGCUGgGUcUACCCAGc -3' miRNA: 3'- aAUGcGUCGACgCAuAUGGGUC- -5' |
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| MIMAT0002185 | kshv-miR-K12-9 | -46.8 | NC_003409.1 | - | 121970 | 0.7 | 0.963459 |
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Target: 5'- uUUACGCAgGgUGCGgugcUGCCCAGg -3' miRNA: 3'- -AAUGCGU-CgACGCau--AUGGGUC- -5' |
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| MIMAT0002185 | kshv-miR-K12-9 | -46.8 | NC_003409.1 | - | 130917 | 0.68 | 0.992032 |
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Target: 5'- -gACGguGCUGCccagcuggGCCCAGg -3' miRNA: 3'- aaUGCguCGACGcaua----UGGGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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