Results 1 - 9 of 9 are showing below:
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| Acc. No. | Name | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| MIMAT0002194 | kshv-miR-K12-3* | -44.5 | NC_003409.1 | - | 15486 | 0.66 | 0.999821 |
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Target: 5'- -cUCACAgUCUGgauaGACUGCGAa -3' miRNA: 3'- acAGUGUaAGACa---CUGGCGCU- -5' |
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| MIMAT0002194 | kshv-miR-K12-3* | -44.5 | NC_003409.1 | + | 32264 | 0.67 | 0.999605 |
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Target: 5'- uUGUUAUuggaagggGUUCUGUGACUGgGAg -3' miRNA: 3'- -ACAGUG--------UAAGACACUGGCgCU- -5' |
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| MIMAT0002194 | kshv-miR-K12-3* | -44.5 | NC_003409.1 | - | 60848 | 0.67 | 0.999765 |
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Target: 5'- gUGcCACGUUCcgGUGgacGCCGCGGg -3' miRNA: 3'- -ACaGUGUAAGa-CAC---UGGCGCU- -5' |
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| MIMAT0002194 | kshv-miR-K12-3* | -44.5 | NC_003409.1 | - | 63518 | 0.68 | 0.998989 |
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Target: 5'- gGUC-CAUUCacguaUGUGAUCGCGGa -3' miRNA: 3'- aCAGuGUAAG-----ACACUGGCGCU- -5' |
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| MIMAT0002194 | kshv-miR-K12-3* | -44.5 | NC_003409.1 | - | 73617 | 0.66 | 0.999821 |
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Target: 5'- --cCAUAUUCUGUGA-CGCGGc -3' miRNA: 3'- acaGUGUAAGACACUgGCGCU- -5' |
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| MIMAT0002194 | kshv-miR-K12-3* | -44.5 | NC_003409.1 | + | 93341 | 0.72 | 0.979321 |
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Target: 5'- -uUCGC--UCUGUGACCGUGAu -3' miRNA: 3'- acAGUGuaAGACACUGGCGCU- -5' |
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| MIMAT0002194 | kshv-miR-K12-3* | -44.5 | NC_003409.1 | - | 113237 | 0.71 | 0.987818 |
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Target: 5'- cGUCGCGg--UGUGGCUGCGAc -3' miRNA: 3'- aCAGUGUaagACACUGGCGCU- -5' |
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| MIMAT0002194 | kshv-miR-K12-3* | -44.5 | NC_003409.1 | + | 116367 | 0.68 | 0.998744 |
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Target: 5'- gGUCGCAg--UG-GACCGCGAg -3' miRNA: 3'- aCAGUGUaagACaCUGGCGCU- -5' |
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| MIMAT0002194 | kshv-miR-K12-3* | -44.5 | NC_003409.1 | + | 121424 | 1.01 | 0.060773 |
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Target: 5'- gUGUCACAUUCUGUGACCGCGAc -3' miRNA: 3'- -ACAGUGUAAGACACUGGCGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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