miRNA display CGI


Results 1 - 16 of 16 are showing below:
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Acc. No. Name Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
MIMAT0003341 hcmv-miR-US4 -51.2 NC_006273.1 - 201427 1.01 0.025649
Target:  5'- cAUCCCCCUGCACGUCCAUGUCGc -3'
miRNA:   3'- -UAGGGGGACGUGCAGGUACAGC- -5'
MIMAT0003341 hcmv-miR-US4 -51.2 NC_006273.1 - 104731 0.71 0.877786
Target:  5'- uUCCCCCUGCugGUCaaacaccagGUCu -3'
miRNA:   3'- uAGGGGGACGugCAGgua------CAGc -5'
MIMAT0003341 hcmv-miR-US4 -51.2 NC_006273.1 + 117357 0.71 0.882959
Target:  5'- uUCUCCCUcggagccggcagagGCgGCGUCCAUGUCGc -3'
miRNA:   3'- uAGGGGGA--------------CG-UGCAGGUACAGC- -5'
MIMAT0003341 hcmv-miR-US4 -51.2 NC_006273.1 + 33361 0.7 0.923981
Target:  5'- gAUCCUgCgGCGCGUCCccGUGUCGc -3'
miRNA:   3'- -UAGGGgGaCGUGCAGG--UACAGC- -5'
MIMAT0003341 hcmv-miR-US4 -51.2 NC_006273.1 + 52337 0.7 0.929549
Target:  5'- aGUCCCCC--CACGUCCAucugcgucUGUCGc -3'
miRNA:   3'- -UAGGGGGacGUGCAGGU--------ACAGC- -5'
MIMAT0003341 hcmv-miR-US4 -51.2 NC_006273.1 + 102113 0.7 0.934856
Target:  5'- --gCCgCUGCAaGUCCGUGUCGg -3'
miRNA:   3'- uagGGgGACGUgCAGGUACAGC- -5'
MIMAT0003341 hcmv-miR-US4 -51.2 NC_006273.1 - 95931 0.69 0.94922
Target:  5'- gAUCgCCCCUGC-CGUCauauUGUCGu -3'
miRNA:   3'- -UAG-GGGGACGuGCAGgu--ACAGC- -5'
MIMAT0003341 hcmv-miR-US4 -51.2 NC_006273.1 + 88026 0.68 0.974098
Target:  5'- cUCCCCCccgGCgGCGUCCGUuaaucgcugggcGUCGg -3'
miRNA:   3'- uAGGGGGa--CG-UGCAGGUA------------CAGC- -5'
MIMAT0003341 hcmv-miR-US4 -51.2 NC_006273.1 - 131779 0.68 0.974098
Target:  5'- gGUCCUCCagcccaUGCACGUCCAg--CGa -3'
miRNA:   3'- -UAGGGGG------ACGUGCAGGUacaGC- -5'
MIMAT0003341 hcmv-miR-US4 -51.2 NC_006273.1 + 209238 0.67 0.976746
Target:  5'- uUCCCgCUGCGUGgaaacugucUCCAUGUCGg -3'
miRNA:   3'- uAGGGgGACGUGC---------AGGUACAGC- -5'
MIMAT0003341 hcmv-miR-US4 -51.2 NC_006273.1 + 129616 0.67 0.985382
Target:  5'- cGUCCCCgUGCACGUUCGc---- -3'
miRNA:   3'- -UAGGGGgACGUGCAGGUacagc -5'
MIMAT0003341 hcmv-miR-US4 -51.2 NC_006273.1 + 178482 0.66 0.990057
Target:  5'- -gCCgCCaCUGU-CGUCCGUGUCGu -3'
miRNA:   3'- uaGG-GG-GACGuGCAGGUACAGC- -5'
MIMAT0003341 hcmv-miR-US4 -51.2 NC_006273.1 - 185270 0.66 0.990057
Target:  5'- -aUCCCCUGCACGgUCAUGa-- -3'
miRNA:   3'- uaGGGGGACGUGCaGGUACagc -5'
MIMAT0003341 hcmv-miR-US4 -51.2 NC_006273.1 - 119274 0.66 0.992457
Target:  5'- -cCCCCCUGUcCGaagCCAUGUUu -3'
miRNA:   3'- uaGGGGGACGuGCa--GGUACAGc -5'
MIMAT0003341 hcmv-miR-US4 -51.2 NC_006273.1 - 54560 0.66 0.992457
Target:  5'- aAUUCCCCUGCcuACGUCUucAUGaCGg -3'
miRNA:   3'- -UAGGGGGACG--UGCAGG--UACaGC- -5'
MIMAT0003341 hcmv-miR-US4 -51.2 NC_006273.1 - 119132 0.66 0.99347
Target:  5'- cAUCCCCUcgugGCACGUCUuUG-CGa -3'
miRNA:   3'- -UAGGGGGa---CGUGCAGGuACaGC- -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.