miRNA: 3'- -UUCCGAGUAAAGUCCGGGGAGU- -5'
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| Acc. No. | Name | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| MIMAT0003344 | sv40-miR-S1-5p | -49 | NC_001669.1 | - | 2818 | 1.02 | 0.000225 |
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Target: 5'- cAAGGCUCAUUUCAGGCCCCUCAg -3' miRNA: 3'- -UUCCGAGUAAAGUCCGGGGAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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