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Acc. No. | Name | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
MIMAT0003431 | rlcv-miR-rL1-4-3p | -47.6 | NC_006146.1 | - | 144545 | 0.66 | 0.999073 |
Target: 5'- -aGCCUGGUGGG-CGUaGUGGg- -3' miRNA: 3'- ucUGGAUCACCUaGCA-CACCac -5' |
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MIMAT0003431 | rlcv-miR-rL1-4-3p | -47.6 | NC_006146.1 | - | 52327 | 0.67 | 0.997016 |
Target: 5'- cGACCUGGUGGGgccCGaGcUGGUGu -3' miRNA: 3'- uCUGGAUCACCUa--GCaC-ACCAC- -5' |
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MIMAT0003431 | rlcv-miR-rL1-4-3p | -47.6 | NC_006146.1 | + | 60140 | 0.71 | 0.953203 |
Target: 5'- gGGACCUGGUGGGggGUGU-GUGc -3' miRNA: 3'- -UCUGGAUCACCUagCACAcCAC- -5' |
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MIMAT0003431 | rlcv-miR-rL1-4-3p | -47.6 | NC_006146.1 | - | 133386 | 1.02 | 0.036983 |
Target: 5'- gAGACCUAGUGGAUCGUGUGGUGu -3' miRNA: 3'- -UCUGGAUCACCUAGCACACCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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