miRNA: 3'- -UCGUGUA-GUGG-CCGugAUCCaa -5'
miRNA: 3'- -UCGUGuAGUGGCCGUgaUCCAa -5'
miRNA: 3'- -UCGUGUAGUGGCCGUGAUCCAA- -5'
Results 21 - 23 of 23 are showing below:
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| Acc. No. | Name | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| MIMAT0003432 | rlcv-miR-rL1-5-5p | -48.3 | NC_006146.1 | + | 156767 | 0.72 | 0.89525 |
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Target: 5'- cAGCGCGUaCACCuGGCugUAGGc- -3' miRNA: 3'- -UCGUGUA-GUGG-CCGugAUCCaa -5' |
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| MIMAT0003432 | rlcv-miR-rL1-5-5p | -48.3 | NC_006146.1 | + | 41699 | 0.75 | 0.790129 |
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Target: 5'- cAGCGC-UCACCGGCGggGGGUg -3' miRNA: 3'- -UCGUGuAGUGGCCGUgaUCCAa -5' |
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| MIMAT0003432 | rlcv-miR-rL1-5-5p | -48.3 | NC_006146.1 | - | 133619 | 1 | 0.034172 |
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Target: 5'- cAGCACAUCACCGGCACUAGGUUc -3' miRNA: 3'- -UCGUGUAGUGGCCGUGAUCCAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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