miRNA: 3'- -UCGUGuAG----UGGCCGUGAUCCaa -5'
miRNA: 3'- -UCGUGuAGUGGCCGUgaUCCAa -5'
miRNA: 3'- -UCGUGua----GUGgCCGUGAUCCaa -5'
Results 21 - 23 of 23 are showing below:
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| Acc. No. | Name | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| MIMAT0003432 | rlcv-miR-rL1-5-5p | -48.3 | NC_006146.1 | + | 44900 | 0.69 | 0.974931 |
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Target: 5'- uGGUACcUUgacgGCCGGCGCUGGGa- -3' miRNA: 3'- -UCGUGuAG----UGGCCGUGAUCCaa -5' |
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| MIMAT0003432 | rlcv-miR-rL1-5-5p | -48.3 | NC_006146.1 | + | 41699 | 0.75 | 0.790129 |
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Target: 5'- cAGCGC-UCACCGGCGggGGGUg -3' miRNA: 3'- -UCGUGuAGUGGCCGUgaUCCAa -5' |
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| MIMAT0003432 | rlcv-miR-rL1-5-5p | -48.3 | NC_006146.1 | + | 41207 | 0.66 | 0.995884 |
|
Target: 5'- aGGCGCcccaucCGCgGGCACUGGGc- -3' miRNA: 3'- -UCGUGua----GUGgCCGUGAUCCaa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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