Results 1 - 11 of 11 are showing below:
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| Acc. No. | Name | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| MIMAT0003433 | rlcv-miR-rL1-5-3p | -44.5 | NC_006146.1 | + | 121553 | 0.66 | 0.99999 |
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Target: 5'- -aGCCUaucaucgaGGUGAAAAGUGG-GCc -3' miRNA: 3'- ugUGGA--------UCACUUUUCACCaCGc -5' |
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| MIMAT0003433 | rlcv-miR-rL1-5-3p | -44.5 | NC_006146.1 | + | 44829 | 0.66 | 0.999961 |
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Target: 5'- uGCGgCUGGUGAcgGAGGUGgGUGUa -3' miRNA: 3'- -UGUgGAUCACU--UUUCAC-CACGc -5' |
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| MIMAT0003433 | rlcv-miR-rL1-5-3p | -44.5 | NC_006146.1 | + | 103838 | 0.67 | 0.999928 |
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Target: 5'- -aACCUGG-GAGAAGagGGUGCu -3' miRNA: 3'- ugUGGAUCaCUUUUCa-CCACGc -5' |
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| MIMAT0003433 | rlcv-miR-rL1-5-3p | -44.5 | NC_006146.1 | - | 144545 | 0.67 | 0.999928 |
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Target: 5'- -aGCCUGGUGGGcguAGUGGgGCa -3' miRNA: 3'- ugUGGAUCACUUu--UCACCaCGc -5' |
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| MIMAT0003433 | rlcv-miR-rL1-5-3p | -44.5 | NC_006146.1 | + | 105753 | 0.68 | 0.999627 |
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Target: 5'- cACACCUGGUGA--GGUG-UGCc -3' miRNA: 3'- -UGUGGAUCACUuuUCACcACGc -5' |
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| MIMAT0003433 | rlcv-miR-rL1-5-3p | -44.5 | NC_006146.1 | - | 133800 | 0.68 | 0.999524 |
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Target: 5'- aGC-CCUGGUGAGAcauAGUGGauaGCGg -3' miRNA: 3'- -UGuGGAUCACUUU---UCACCa--CGC- -5' |
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| MIMAT0003433 | rlcv-miR-rL1-5-3p | -44.5 | NC_006146.1 | + | 81063 | 0.68 | 0.999501 |
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Target: 5'- cGCGCCacaaaguguuggGGUGccAAGUGGUGCGu -3' miRNA: 3'- -UGUGGa-----------UCACuuUUCACCACGC- -5' |
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| MIMAT0003433 | rlcv-miR-rL1-5-3p | -44.5 | NC_006146.1 | + | 131535 | 0.71 | 0.99473 |
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Target: 5'- gGCGCCgAGUGGGAcguuuGGUGGUGgGa -3' miRNA: 3'- -UGUGGaUCACUUU-----UCACCACgC- -5' |
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| MIMAT0003433 | rlcv-miR-rL1-5-3p | -44.5 | NC_006146.1 | - | 133384 | 0.72 | 0.992744 |
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Target: 5'- -gACCUAGUGGAucguGUGGUGUu -3' miRNA: 3'- ugUGGAUCACUUuu--CACCACGc -5' |
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| MIMAT0003433 | rlcv-miR-rL1-5-3p | -44.5 | NC_006146.1 | + | 60142 | 0.77 | 0.908387 |
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Target: 5'- -gACCUGGUGggGGGUGuGUGCc -3' miRNA: 3'- ugUGGAUCACuuUUCAC-CACGc -5' |
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| MIMAT0003433 | rlcv-miR-rL1-5-3p | -44.5 | NC_006146.1 | - | 133657 | 1.01 | 0.074698 |
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Target: 5'- gACACCUAGUGAAAAGUGGUGCGc -3' miRNA: 3'- -UGUGGAUCACUUUUCACCACGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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