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| Acc. No. | Name | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| MIMAT0003434 | rlcv-miR-rL1-6-5p | -45.8 | NC_006146.1 | - | 56680 | 0.66 | 0.99979 |
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Target: 5'- gACuGCGCG-CGCUgaugCCGCUGGAg -3' miRNA: 3'- gUGuCGUGCaGUGAa---GGUGAUUU- -5' |
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| MIMAT0003434 | rlcv-miR-rL1-6-5p | -45.8 | NC_006146.1 | - | 120662 | 0.66 | 0.999878 |
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Target: 5'- gCGCAGCgACGUCAUggCCAaUAGAa -3' miRNA: 3'- -GUGUCG-UGCAGUGaaGGUgAUUU- -5' |
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| MIMAT0003434 | rlcv-miR-rL1-6-5p | -45.8 | NC_006146.1 | - | 151649 | 0.66 | 0.99979 |
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Target: 5'- aCGCGGCGCGcCGCUUCgucCUGGAc -3' miRNA: 3'- -GUGUCGUGCaGUGAAGgu-GAUUU- -5' |
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| MIMAT0003434 | rlcv-miR-rL1-6-5p | -45.8 | NC_006146.1 | + | 91507 | 0.67 | 0.999727 |
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Target: 5'- -uCAGCugGUCAUUagCCGCUGu- -3' miRNA: 3'- guGUCGugCAGUGAa-GGUGAUuu -5' |
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| MIMAT0003434 | rlcv-miR-rL1-6-5p | -45.8 | NC_006146.1 | + | 104850 | 0.67 | 0.999649 |
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Target: 5'- uCACAGCGCGUCAgCUccUCCGg---- -3' miRNA: 3'- -GUGUCGUGCAGU-GA--AGGUgauuu -5' |
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| MIMAT0003434 | rlcv-miR-rL1-6-5p | -45.8 | NC_006146.1 | + | 151465 | 0.69 | 0.99706 |
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Target: 5'- aGCGGCGCGcCGCgUCCACg--- -3' miRNA: 3'- gUGUCGUGCaGUGaAGGUGauuu -5' |
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| MIMAT0003434 | rlcv-miR-rL1-6-5p | -45.8 | NC_006146.1 | - | 30664 | 0.69 | 0.997555 |
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Target: 5'- uUACAGCAUGUCACacgCCagcACUGAGu -3' miRNA: 3'- -GUGUCGUGCAGUGaa-GG---UGAUUU- -5' |
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| MIMAT0003434 | rlcv-miR-rL1-6-5p | -45.8 | NC_006146.1 | - | 133756 | 1.01 | 0.056751 |
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Target: 5'- uCACAGCACGUCACUUCCACUAAAa -3' miRNA: 3'- -GUGUCGUGCAGUGAAGGUGAUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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