miRNA: 3'- -GGGGCauaGGA-----UAGCUCACAGu -5'
miRNA: 3'- -GGGGCAUAGGAUAGcUCACAgu -5'
miRNA: 3'- -GGGGCAUAGGAUAGCUCACAGU- -5'
Results 1 - 3 of 3 are showing below:
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| Acc. No. | Name | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| MIMAT0003440 | rlcv-miR-rL1-11 | -47.4 | NC_006146.1 | - | 151476 | 0.67 | 0.997164 |
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Target: 5'- gCCCUGg--CCUucgauAUCGAGUGUCu -3' miRNA: 3'- -GGGGCauaGGA-----UAGCUCACAGu -5' |
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| MIMAT0003440 | rlcv-miR-rL1-11 | -47.4 | NC_006146.1 | + | 141206 | 0.74 | 0.882857 |
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Target: 5'- cCUCCGUAUCCUAUCaAGUGUg- -3' miRNA: 3'- -GGGGCAUAGGAUAGcUCACAgu -5' |
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| MIMAT0003440 | rlcv-miR-rL1-11 | -47.4 | NC_006146.1 | - | 141435 | 1.01 | 0.042357 |
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Target: 5'- cCCCCGUAUCCUAUCGAGUGUCAc -3' miRNA: 3'- -GGGGCAUAGGAUAGCUCACAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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