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Acc. No. | Name | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
MIMAT0003441 | rlcv-miR-rL1-12-5p | -50.2 | NC_006146.1 | - | 106247 | 0.67 | 0.972274 |
Target: 5'- gCCGCcucagGUGCAgaagGGUCgGGUCUg -3' miRNA: 3'- gGGUGa----CACGUa---CCAGaCCAGA- -5' |
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MIMAT0003441 | rlcv-miR-rL1-12-5p | -50.2 | NC_006146.1 | + | 73080 | 0.7 | 0.90452 |
Target: 5'- aCCACUG-GCAUGGagUGGUUg -3' miRNA: 3'- gGGUGACaCGUACCagACCAGa -5' |
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MIMAT0003441 | rlcv-miR-rL1-12-5p | -50.2 | NC_006146.1 | - | 107456 | 0.78 | 0.504169 |
Target: 5'- uCCCGCUGUGCcugaaggugAUGGUgCUGGUCUc -3' miRNA: 3'- -GGGUGACACG---------UACCA-GACCAGA- -5' |
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MIMAT0003441 | rlcv-miR-rL1-12-5p | -50.2 | NC_006146.1 | - | 141608 | 1.01 | 0.019658 |
Target: 5'- gCCCACUGUGCAUGGUCUGGUCUg -3' miRNA: 3'- -GGGUGACACGUACCAGACCAGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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