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| Acc. No. | Name | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| MIMAT0003448 | rlcv-miR-rL1-16-3p | -44.3 | NC_006146.1 | + | 154528 | 0.66 | 0.999977 |
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Target: 5'- -cAACAGGCCcucuGUGUGUUUCu-- -3' miRNA: 3'- ccUUGUCCGG----UACACAAAGuac -5' |
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| MIMAT0003448 | rlcv-miR-rL1-16-3p | -44.3 | NC_006146.1 | + | 167577 | 0.66 | 0.999984 |
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Target: 5'- cGGAgAGGCCGUGUGUggaggCcgGg -3' miRNA: 3'- cCUUgUCCGGUACACAaa---GuaC- -5' |
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| MIMAT0003448 | rlcv-miR-rL1-16-3p | -44.3 | NC_006146.1 | + | 168509 | 0.66 | 0.999984 |
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Target: 5'- cGGAgAGGCCGUGUGUggaggCcgGg -3' miRNA: 3'- cCUUgUCCGGUACACAaa---GuaC- -5' |
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| MIMAT0003448 | rlcv-miR-rL1-16-3p | -44.3 | NC_006146.1 | + | 169441 | 0.66 | 0.999984 |
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Target: 5'- cGGAgAGGCCGUGUGUggaggCcgGg -3' miRNA: 3'- cCUUgUCCGGUACACAaa---GuaC- -5' |
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| MIMAT0003448 | rlcv-miR-rL1-16-3p | -44.3 | NC_006146.1 | + | 170372 | 0.66 | 0.999984 |
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Target: 5'- cGGAgAGGCCGUGUGUggaggCcgGg -3' miRNA: 3'- cCUUgUCCGGUACACAaa---GuaC- -5' |
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| MIMAT0003448 | rlcv-miR-rL1-16-3p | -44.3 | NC_006146.1 | - | 150207 | 0.7 | 0.998023 |
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Target: 5'- uGGAGCGGGCCAaGgccUUCGUGg -3' miRNA: 3'- -CCUUGUCCGGUaCacaAAGUAC- -5' |
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| MIMAT0003448 | rlcv-miR-rL1-16-3p | -44.3 | NC_006146.1 | - | 68201 | 0.71 | 0.995074 |
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Target: 5'- gGGGACgccagGGGCCGUGUGg--CGUGg -3' miRNA: 3'- -CCUUG-----UCCGGUACACaaaGUAC- -5' |
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| MIMAT0003448 | rlcv-miR-rL1-16-3p | -44.3 | NC_006146.1 | - | 143827 | 1.01 | 0.077542 |
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Target: 5'- aGGAACAGGCCAUGUGUUUCAUGc -3' miRNA: 3'- -CCUUGUCCGGUACACAAAGUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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