Results 1 - 13 of 13 are showing below:
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Acc. No. | Name | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
MIMAT0003712 | kshv-miR-K12-12 | -51.8 | NC_003409.1 | + | 23974 | 0.66 | 0.937462 |
Target: 5'- gCGGGGGGGGggcuaGUGaGUcacgGGCCUGGa -3' miRNA: 3'- -GCCUCUCCU-----UAC-CA----CCGGACCa -5' |
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MIMAT0003712 | kshv-miR-K12-12 | -51.8 | NC_003409.1 | - | 108814 | 0.66 | 0.950561 |
Target: 5'- uGGAGuGuGGAUGGUgauauggucuccugGGCCUGGc -3' miRNA: 3'- gCCUCuC-CUUACCA--------------CCGGACCa -5' |
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MIMAT0003712 | kshv-miR-K12-12 | -51.8 | NC_003409.1 | + | 108528 | 0.67 | 0.907832 |
Target: 5'- aGGGGGGGAAggccgcGGUGGaauCCUGGc -3' miRNA: 3'- gCCUCUCCUUa-----CCACC---GGACCa -5' |
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MIMAT0003712 | kshv-miR-K12-12 | -51.8 | NC_003409.1 | - | 66649 | 0.67 | 0.901071 |
Target: 5'- gGGuGGGGGAUGGUGGgaggCUGGc -3' miRNA: 3'- gCCuCUCCUUACCACCg---GACCa -5' |
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MIMAT0003712 | kshv-miR-K12-12 | -51.8 | NC_003409.1 | - | 76376 | 0.67 | 0.920524 |
Target: 5'- uCGGucAGuucuGUGGUGGCCUGGa -3' miRNA: 3'- -GCCucUCcu--UACCACCGGACCa -5' |
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MIMAT0003712 | kshv-miR-K12-12 | -51.8 | NC_003409.1 | - | 117736 | 0.68 | 0.894036 |
Target: 5'- uCGGuGGGGGAgGGUGcCCUGGUu -3' miRNA: 3'- -GCCuCUCCUUaCCACcGGACCA- -5' |
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MIMAT0003712 | kshv-miR-K12-12 | -51.8 | NC_003409.1 | + | 48971 | 0.7 | 0.790564 |
Target: 5'- --cGGAGGAAUGGUGGacggcuccguCCUGGUg -3' miRNA: 3'- gccUCUCCUUACCACC----------GGACCA- -5' |
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MIMAT0003712 | kshv-miR-K12-12 | -51.8 | NC_003409.1 | + | 118301 | 0.7 | 0.780612 |
Target: 5'- cCGGGGAGGAGgcuggGGUGcGCCgcgccgGGUu -3' miRNA: 3'- -GCCUCUCCUUa----CCAC-CGGa-----CCA- -5' |
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MIMAT0003712 | kshv-miR-K12-12 | -51.8 | NC_003409.1 | + | 12194 | 0.71 | 0.760252 |
Target: 5'- cCGGAGAGGAcgGaGUGGCCc--- -3' miRNA: 3'- -GCCUCUCCUuaC-CACCGGacca -5' |
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MIMAT0003712 | kshv-miR-K12-12 | -51.8 | NC_003409.1 | - | 22757 | 0.71 | 0.746726 |
Target: 5'- uGGGGAGGAAaagcgcggcaccccUGGUcGCCUGGg -3' miRNA: 3'- gCCUCUCCUU--------------ACCAcCGGACCa -5' |
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MIMAT0003712 | kshv-miR-K12-12 | -51.8 | NC_003409.1 | - | 87917 | 0.73 | 0.61907 |
Target: 5'- gGGAGAucuuccugucacGGGAUGGUGGCCUcaGGg -3' miRNA: 3'- gCCUCU------------CCUUACCACCGGA--CCa -5' |
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MIMAT0003712 | kshv-miR-K12-12 | -51.8 | NC_003409.1 | - | 90959 | 0.74 | 0.585917 |
Target: 5'- uGGGGAGGggUGG-GGCaaaUGGUu -3' miRNA: 3'- gCCUCUCCuuACCaCCGg--ACCA- -5' |
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MIMAT0003712 | kshv-miR-K12-12 | -51.8 | NC_003409.1 | + | 117611 | 1 | 0.014084 |
Target: 5'- gCGGAGAGGAAUGGUGGCCUGGUu -3' miRNA: 3'- -GCCUCUCCUUACCACCGGACCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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