miRNA: 3'- gauAGUUCCU---UUGUUUUGGUGa -5'
miRNA: 3'- gaUAGUUCCUUUGUU-UUGGUGa -5'
miRNA: 3'- -GAUAGUUCCUUUGUUUUGGUGA- -5'
Results 41 - 43 of 43 are showing below:
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| Acc. No. | Name | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| MIMAT0003713 | ebv-miR-BART15 | -38.4 | NC_007605.1 | + | 95043 | 0.74 | 0.999988 |
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Target: 5'- --cUCAGGGGccaAGCAAAACCGCg -3' miRNA: 3'- gauAGUUCCU---UUGUUUUGGUGa -5' |
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| MIMAT0003713 | ebv-miR-BART15 | -38.4 | NC_007605.1 | - | 117664 | 0.81 | 0.992902 |
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Target: 5'- -gAUCGAGGAGACGAgGGCCACg -3' miRNA: 3'- gaUAGUUCCUUUGUU-UUGGUGa -5' |
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| MIMAT0003713 | ebv-miR-BART15 | -38.4 | NC_007605.1 | - | 139610 | 1.02 | 0.296642 |
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Target: 5'- uCUAUCAAGGAAACAAAACCACUg -3' miRNA: 3'- -GAUAGUUCCUUUGUUUUGGUGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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