miRNA: 3'- -GUUCUCGUGUGUgGGUGAGaua -5'
miRNA: 3'- gUUCUCGUGUG-----UGGGUGAGaua -5'
miRNA: 3'- gUUCUCGUGuGUGGGUGAgaua -5'
miRNA: 3'- gUUCUCGUgUGUGGgUGAGAUA- -5'
miRNA: 3'- -GUUCUCGUGUGUGGGUGAGAUA- -5'
Results 21 - 25 of 25 are showing below:
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| Acc. No. | Name | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| MIMAT0003714 | ebv-miR-BART16 | -45.8 | NC_007605.1 | + | 116394 | 0.72 | 0.978726 |
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Target: 5'- gUAAGGGCGCACA-CCACUCc-- -3' miRNA: 3'- -GUUCUCGUGUGUgGGUGAGaua -5' |
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| MIMAT0003714 | ebv-miR-BART16 | -45.8 | NC_007605.1 | - | 55020 | 0.72 | 0.973135 |
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Target: 5'- uGGGGGCACGCccagaGCCCGCUCc-- -3' miRNA: 3'- gUUCUCGUGUG-----UGGGUGAGaua -5' |
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| MIMAT0003714 | ebv-miR-BART16 | -45.8 | NC_007605.1 | + | 10470 | 0.72 | 0.966551 |
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Target: 5'- gGAGGGCACuCGCCCACUa--- -3' miRNA: 3'- gUUCUCGUGuGUGGGUGAgaua -5' |
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| MIMAT0003714 | ebv-miR-BART16 | -45.8 | NC_007605.1 | - | 135969 | 0.74 | 0.934781 |
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Target: 5'- gAGGGGCAgGCGCUgACUCUGUg -3' miRNA: 3'- gUUCUCGUgUGUGGgUGAGAUA- -5' |
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| MIMAT0003714 | ebv-miR-BART16 | -45.8 | NC_007605.1 | - | 139853 | 1.01 | 0.05673 |
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Target: 5'- aCAAGAGCACACACCCACUCUAUc -3' miRNA: 3'- -GUUCUCGUGUGUGGGUGAGAUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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