miRNA: 3'- guuCUCGUGUG-----------UGGGUGAgAUA- -5'
miRNA: 3'- -GUUCUCGUGUGUGGGUGAGAUA- -5'
miRNA: 3'- guUCUCG-UGUGUGGGu--GAGAUa -5'
miRNA: 3'- guuCUCG-UGUGUGGGUGa---GAUa -5'
miRNA: 3'- -GUUCUCGUgUGUGGg---UGAGaua -5'
Results 21 - 25 of 25 are showing below:
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| Acc. No. | Name | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| MIMAT0003714 | ebv-miR-BART16 | -45.8 | NC_007605.1 | + | 137056 | 0.67 | 0.999063 |
|
Target: 5'- --cGGGCACACaggcccccgaaGCCCACUgUGUg -3' miRNA: 3'- guuCUCGUGUG-----------UGGGUGAgAUA- -5' |
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| MIMAT0003714 | ebv-miR-BART16 | -45.8 | NC_007605.1 | - | 139853 | 1.01 | 0.05673 |
|
Target: 5'- aCAAGAGCACACACCCACUCUAUc -3' miRNA: 3'- -GUUCUCGUGUGUGGGUGAGAUA- -5' |
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| MIMAT0003714 | ebv-miR-BART16 | -45.8 | NC_007605.1 | - | 150650 | 0.7 | 0.992875 |
|
Target: 5'- -cAGGGCgGCACGCCCucuCUCUGg -3' miRNA: 3'- guUCUCG-UGUGUGGGu--GAGAUa -5' |
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| MIMAT0003714 | ebv-miR-BART16 | -45.8 | NC_007605.1 | - | 163476 | 0.67 | 0.999403 |
|
Target: 5'- --uGAGCuACGCACCCGCcaugCUGg -3' miRNA: 3'- guuCUCG-UGUGUGGGUGa---GAUa -5' |
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| MIMAT0003714 | ebv-miR-BART16 | -45.8 | NC_007605.1 | - | 168207 | 0.68 | 0.998839 |
|
Target: 5'- aCGAGGGCAgACACCaccuGCUCg-- -3' miRNA: 3'- -GUUCUCGUgUGUGGg---UGAGaua -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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