miRNA display CGI

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Acc. No.	Name	Perfect MFE.*	RefSeq Acc.	strand	Start Position	R_P_ratio#	P value
	Predicted miRNA align pattern
MIMAT0003720	ebv-miR-BART20-3p	-46.7	NC_007605.1	+	85451	0.66	0.999249
	Target:  5'- ---uGCGGGCUGgcaGCCUUCAc- -3' miRNA:   3'- ccauUGUCCGACa--CGGAAGUac -5'
MIMAT0003720	ebv-miR-BART20-3p	-46.7	NC_007605.1	-	154162	0.68	0.99391
	Target:  5'- uGG-AGCGGGCcaagGCCUUCGUGg -3' miRNA:   3'- -CCaUUGUCCGaca-CGGAAGUAC- -5'
MIMAT0003720	ebv-miR-BART20-3p	-46.7	NC_007605.1	-	122457	0.69	0.990386
	Target:  5'- uGGUGaACGGGCUGgugGCCUUUg-- -3' miRNA:   3'- -CCAU-UGUCCGACa--CGGAAGuac -5'
MIMAT0003720	ebv-miR-BART20-3p	-46.7	NC_007605.1	+	159156	0.71	0.973135
	Target:  5'- aGGUGuACAGGCUGUGCg-UCAg- -3' miRNA:   3'- -CCAU-UGUCCGACACGgaAGUac -5'
MIMAT0003720	ebv-miR-BART20-3p	-46.7	NC_007605.1	+	55041	0.71	0.966551
	Target:  5'- cGGUGGCGGGCUuaaaGCCUUCGg- -3' miRNA:   3'- -CCAUUGUCCGAca--CGGAAGUac -5'
MIMAT0003720	ebv-miR-BART20-3p	-46.7	NC_007605.1	+	65852	0.77	0.768005
	Target:  5'- aGGUGACGGGCUGUucgacGCCUUCu-- -3' miRNA:   3'- -CCAUUGUCCGACA-----CGGAAGuac -5'
MIMAT0003720	ebv-miR-BART20-3p	-46.7	NC_007605.1	-	148419	1	0.045841
	Target:  5'- uGGUAACAGGCUGUGCCUUCAUGc -3' miRNA:   3'- -CCAUUGUCCGACACGGAAGUAC- -5'

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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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