Results 1 - 9 of 9 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1002 | 3' | -50.1 | NC_000924.1 | + | 32392 | 0.66 | 0.956309 |
Target: 5'- aCga-CCUGAUgGAGCAGAucgaacguccggAUGGCAUUa -3' miRNA: 3'- gGaagGGACUA-UUCGUCU------------UGCCGUAG- -5' |
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1002 | 3' | -50.1 | NC_000924.1 | + | 15429 | 0.66 | 0.93711 |
Target: 5'- uUCUUCCCUGGU-AGCAaacCGGUAa- -3' miRNA: 3'- -GGAAGGGACUAuUCGUcuuGCCGUag -5' |
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1002 | 3' | -50.1 | NC_000924.1 | + | 19774 | 0.67 | 0.931583 |
Target: 5'- uCUUUCUCUGAUGaaGGCGuuACGGCu-- -3' miRNA: 3'- -GGAAGGGACUAU--UCGUcuUGCCGuag -5' |
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1002 | 3' | -50.1 | NC_000924.1 | + | 5792 | 0.67 | 0.906526 |
Target: 5'- uCCgaaaUCCCUGA-AAGCAcAGCGGCu-- -3' miRNA: 3'- -GGa---AGGGACUaUUCGUcUUGCCGuag -5' |
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1002 | 3' | -50.1 | NC_000924.1 | + | 50564 | 0.68 | 0.89953 |
Target: 5'- gCUUCCCggcGAUGAGCugcGGAU-GCGUCa -3' miRNA: 3'- gGAAGGGa--CUAUUCGu--CUUGcCGUAG- -5' |
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1002 | 3' | -50.1 | NC_000924.1 | + | 53625 | 0.68 | 0.86791 |
Target: 5'- --aUCCCUGAcGGGCGGAccguucgcgagaaGCuGCGUCa -3' miRNA: 3'- ggaAGGGACUaUUCGUCU-------------UGcCGUAG- -5' |
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1002 | 3' | -50.1 | NC_000924.1 | + | 57157 | 0.69 | 0.860363 |
Target: 5'- aUCUUCUgCUGGUcuGCAu-ACGGCAUCa -3' miRNA: 3'- -GGAAGG-GACUAuuCGUcuUGCCGUAG- -5' |
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1002 | 3' | -50.1 | NC_000924.1 | + | 35561 | 0.69 | 0.860363 |
Target: 5'- aCCUUugacCCCUGAUAGaaaCGGAuCGGCAUa -3' miRNA: 3'- -GGAA----GGGACUAUUc--GUCUuGCCGUAg -5' |
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1002 | 3' | -50.1 | NC_000924.1 | + | 7152 | 1.13 | 0.00193 |
Target: 5'- gCCUUCCCUGAUAAGCAGAACGGCAUCg -3' miRNA: 3'- -GGAAGGGACUAUUCGUCUUGCCGUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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