Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1003 | 3' | -52.7 | NC_000924.1 | + | 14625 | 0.66 | 0.845497 |
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Target: 5'- cUUUCCCGGcGAGucuggcuaaCCGGGACCagaacgaACUGa -3' miRNA: 3'- aAAAGGGCCuUUC---------GGUCUUGGg------UGAC- -5' |
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| 1003 | 3' | -52.7 | NC_000924.1 | + | 6256 | 0.66 | 0.83651 |
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Target: 5'- gUUUUCCCc--AGGCCAGAGCCUuaGCg- -3' miRNA: 3'- -AAAAGGGccuUUCGGUCUUGGG--UGac -5' |
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| 1003 | 3' | -52.7 | NC_000924.1 | + | 39094 | 0.67 | 0.798399 |
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Target: 5'- --cUCgUGGAGAGCCAGGuCCgGCa- -3' miRNA: 3'- aaaAGgGCCUUUCGGUCUuGGgUGac -5' |
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| 1003 | 3' | -52.7 | NC_000924.1 | + | 52234 | 0.67 | 0.788384 |
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Target: 5'- uUUUUCUCGGAAAGCaGGGAUgaaUUACUGg -3' miRNA: 3'- -AAAAGGGCCUUUCGgUCUUG---GGUGAC- -5' |
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| 1003 | 3' | -52.7 | NC_000924.1 | + | 38809 | 0.67 | 0.778198 |
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Target: 5'- ---gCCCuGcgGGCCcuugAGGGCCCACUGg -3' miRNA: 3'- aaaaGGGcCuuUCGG----UCUUGGGUGAC- -5' |
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| 1003 | 3' | -52.7 | NC_000924.1 | + | 30384 | 0.67 | 0.767852 |
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Target: 5'- uUUUUCCCGuGAcAGCUucAGCCCGCUu -3' miRNA: 3'- -AAAAGGGC-CUuUCGGucUUGGGUGAc -5' |
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| 1003 | 3' | -52.7 | NC_000924.1 | + | 38869 | 0.67 | 0.757358 |
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Target: 5'- ----aCCGGAGAGCgGGGGCCUAaaGg -3' miRNA: 3'- aaaagGGCCUUUCGgUCUUGGGUgaC- -5' |
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| 1003 | 3' | -52.7 | NC_000924.1 | + | 14714 | 0.72 | 0.506855 |
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Target: 5'- --gUCCCGGuuAGCCAGAcucgccgggaaaguuGCCaguaACUGg -3' miRNA: 3'- aaaAGGGCCuuUCGGUCU---------------UGGg---UGAC- -5' |
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| 1003 | 3' | -52.7 | NC_000924.1 | + | 14767 | 1.07 | 0.002364 |
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Target: 5'- gUUUCCCGGAAAGCCAGAACCCACUGg -3' miRNA: 3'- aAAAGGGCCUUUCGGUCUUGGGUGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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