Results 1 - 3 of 3 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1005 | 3' | -55.6 | NC_000924.1 | + | 31236 | 0.68 | 0.573914 |
Target: 5'- uUCUGGCGcGCAucuccUGGGCaAUCAUGUAGc -3' miRNA: 3'- -AGGCCGU-CGUu----ACCCGcUGGUACAUU- -5' |
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1005 | 3' | -55.6 | NC_000924.1 | + | 42279 | 0.68 | 0.562967 |
Target: 5'- uUCCGGCAGCAAccGGGCaaaACUGgcagGUGAu -3' miRNA: 3'- -AGGCCGUCGUUa-CCCGc--UGGUa---CAUU- -5' |
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1005 | 3' | -55.6 | NC_000924.1 | + | 20375 | 1.06 | 0.001295 |
Target: 5'- aUCCGGCAGCAAUGGGCGACCAUGUAAc -3' miRNA: 3'- -AGGCCGUCGUUACCCGCUGGUACAUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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