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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1006 | 3' | -57.6 | NC_000924.1 | + | 45335 | 0.66 | 0.618401 |
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Target: 5'- gACUGGCGCacgaguggcguuacuGCUGGCauugGCCugaaauucugaccuGUAUCCGg -3' miRNA: 3'- -UGGCUGCG---------------UGACCGga--CGG--------------UAUAGGC- -5' |
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| 1006 | 3' | -57.6 | NC_000924.1 | + | 30613 | 0.67 | 0.561577 |
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Target: 5'- aGCgGACGCugaugaauuaucuGCUGGUaugggaaCUGCCGgAUCCGg -3' miRNA: 3'- -UGgCUGCG-------------UGACCG-------GACGGUaUAGGC- -5' |
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| 1006 | 3' | -57.6 | NC_000924.1 | + | 55354 | 0.68 | 0.480983 |
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Target: 5'- uGCCcggGAUGCACgccccaUGGCCUGCCugcuuucacGUCCGc -3' miRNA: 3'- -UGG---CUGCGUG------ACCGGACGGua-------UAGGC- -5' |
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| 1006 | 3' | -57.6 | NC_000924.1 | + | 40828 | 0.7 | 0.378011 |
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Target: 5'- uCCGcUGUACUGGCggaggaCUGCCAUuUCCGg -3' miRNA: 3'- uGGCuGCGUGACCG------GACGGUAuAGGC- -5' |
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| 1006 | 3' | -57.6 | NC_000924.1 | + | 38110 | 0.74 | 0.203822 |
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Target: 5'- uGCCGACGCACUGGUgccgCUGagCGcAUCCGg -3' miRNA: 3'- -UGGCUGCGUGACCG----GACg-GUaUAGGC- -5' |
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| 1006 | 3' | -57.6 | NC_000924.1 | + | 34797 | 0.75 | 0.192391 |
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Target: 5'- aACCGGCGCacccgcGCUGGUCUGCUucgugcuuuucuUGUCCGg -3' miRNA: 3'- -UGGCUGCG------UGACCGGACGGu-----------AUAGGC- -5' |
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| 1006 | 3' | -57.6 | NC_000924.1 | + | 24075 | 1.07 | 0.000861 |
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Target: 5'- cACCGACGCACUGGCCUGCCAUAUCCGc -3' miRNA: 3'- -UGGCUGCGUGACCGGACGGUAUAGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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