Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1006 | 5' | -48.7 | NC_000924.1 | + | 24487 | 0.66 | 0.972223 |
Target: 5'- aCAgGGAug--GUCAGCCAGUgGCGgCAg -3' miRNA: 3'- -GUgCCUuaaaUAGUUGGUCG-CGUgGU- -5' |
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1006 | 5' | -48.7 | NC_000924.1 | + | 61266 | 0.66 | 0.972223 |
Target: 5'- uCugGaGGAUUUGUCAAUCAGCGgAa-- -3' miRNA: 3'- -GugC-CUUAAAUAGUUGGUCGCgUggu -5' |
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1006 | 5' | -48.7 | NC_000924.1 | + | 53680 | 0.66 | 0.968976 |
Target: 5'- cCAUGGAGcaugUUGUCAcACCAGCGgaaUAUCAg -3' miRNA: 3'- -GUGCCUUa---AAUAGU-UGGUCGC---GUGGU- -5' |
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1006 | 5' | -48.7 | NC_000924.1 | + | 54292 | 0.66 | 0.967951 |
Target: 5'- uCGCGGGAaauaaccagcggCAAauCCGGUGCACCAa -3' miRNA: 3'- -GUGCCUUaaaua-------GUU--GGUCGCGUGGU- -5' |
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1006 | 5' | -48.7 | NC_000924.1 | + | 25419 | 0.66 | 0.965466 |
Target: 5'- aGCGGcccuUUUGUCAAUCuGUcuGCGCCAg -3' miRNA: 3'- gUGCCuu--AAAUAGUUGGuCG--CGUGGU- -5' |
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1006 | 5' | -48.7 | NC_000924.1 | + | 21891 | 0.67 | 0.961687 |
Target: 5'- uCAUGGAAaccgUUGUCAcACCGG-GCACUg -3' miRNA: 3'- -GUGCCUUa---AAUAGU-UGGUCgCGUGGu -5' |
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1006 | 5' | -48.7 | NC_000924.1 | + | 54784 | 0.67 | 0.961687 |
Target: 5'- -cUGGAAUUUAUCcugcauCUGGCGCACa- -3' miRNA: 3'- guGCCUUAAAUAGuu----GGUCGCGUGgu -5' |
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1006 | 5' | -48.7 | NC_000924.1 | + | 24965 | 0.67 | 0.961687 |
Target: 5'- aGCGGAA------AGCCAGCGUauGCCAu -3' miRNA: 3'- gUGCCUUaaauagUUGGUCGCG--UGGU- -5' |
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1006 | 5' | -48.7 | NC_000924.1 | + | 48332 | 0.67 | 0.953289 |
Target: 5'- --aGGAuuggcagUAUCGuACCAGCGCAgCAg -3' miRNA: 3'- gugCCUuaa----AUAGU-UGGUCGCGUgGU- -5' |
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1006 | 5' | -48.7 | NC_000924.1 | + | 16643 | 0.68 | 0.938507 |
Target: 5'- gACGGuggcgUUGUCAugcuugGCguGCGUACCAg -3' miRNA: 3'- gUGCCuua--AAUAGU------UGguCGCGUGGU- -5' |
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1006 | 5' | -48.7 | NC_000924.1 | + | 31044 | 0.68 | 0.938507 |
Target: 5'- gACGGG--UUGUCAGCCagccaaggcggGGCGUAUCGu -3' miRNA: 3'- gUGCCUuaAAUAGUUGG-----------UCGCGUGGU- -5' |
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1006 | 5' | -48.7 | NC_000924.1 | + | 24008 | 0.68 | 0.938507 |
Target: 5'- uGCGGcgug-AUUuACCGGCGUACCGc -3' miRNA: 3'- gUGCCuuaaaUAGuUGGUCGCGUGGU- -5' |
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1006 | 5' | -48.7 | NC_000924.1 | + | 57833 | 0.72 | 0.785074 |
Target: 5'- gACGGggUUUua----CAGCGCACCGg -3' miRNA: 3'- gUGCCuuAAAuaguugGUCGCGUGGU- -5' |
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1006 | 5' | -48.7 | NC_000924.1 | + | 52936 | 0.73 | 0.73138 |
Target: 5'- aAUGGAAccauUCGugcGCCAGCGCACCc -3' miRNA: 3'- gUGCCUUaaauAGU---UGGUCGCGUGGu -5' |
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1006 | 5' | -48.7 | NC_000924.1 | + | 27693 | 0.75 | 0.6059 |
Target: 5'- uCACGGuaauGGUUUGUugcCAGCCacAGCGCACCAu -3' miRNA: 3'- -GUGCC----UUAAAUA---GUUGG--UCGCGUGGU- -5' |
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1006 | 5' | -48.7 | NC_000924.1 | + | 34805 | 0.75 | 0.594413 |
Target: 5'- aUACGGA------CAACCGGCGCACCc -3' miRNA: 3'- -GUGCCUuaaauaGUUGGUCGCGUGGu -5' |
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1006 | 5' | -48.7 | NC_000924.1 | + | 24040 | 1.1 | 0.004652 |
Target: 5'- gCACGGAAUUUAUCAACCAGCGCACCAa -3' miRNA: 3'- -GUGCCUUAAAUAGUUGGUCGCGUGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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