miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
1007 5' -51.2 NC_000924.1 + 55251 0.66 0.920152
Target:  5'- cGGAcgugaaAGCAgGCAGGCCAUggggCGUGCAu -3'
miRNA:   3'- -UCUaug---UCGUgUGUCUGGUG----GCAUGU- -5'
1007 5' -51.2 NC_000924.1 + 51582 0.67 0.87774
Target:  5'- ---cACAGCAgGCAGAaguaCGCCG-ACAg -3'
miRNA:   3'- ucuaUGUCGUgUGUCUg---GUGGCaUGU- -5'
1007 5' -51.2 NC_000924.1 + 16151 0.67 0.87774
Target:  5'- aAGAacACGGuCACGCGGAUagCAUCGUGCGg -3'
miRNA:   3'- -UCUa-UGUC-GUGUGUCUG--GUGGCAUGU- -5'
1007 5' -51.2 NC_000924.1 + 7892 0.72 0.633729
Target:  5'- cGGAaggGCAGCcaGCaACAGGCCACCcUGCAa -3'
miRNA:   3'- -UCUa--UGUCG--UG-UGUCUGGUGGcAUGU- -5'
1007 5' -51.2 NC_000924.1 + 36094 0.74 0.500597
Target:  5'- cGGuUGCAGUACcCAGACCACCcGUugAa -3'
miRNA:   3'- -UCuAUGUCGUGuGUCUGGUGG-CAugU- -5'
1007 5' -51.2 NC_000924.1 + 25356 1.1 0.002489
Target:  5'- gAGAUACAGCACACAGACCACCGUACAg -3'
miRNA:   3'- -UCUAUGUCGUGUGUCUGGUGGCAUGU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.