Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1007 | 5' | -51.2 | NC_000924.1 | + | 55251 | 0.66 | 0.920152 |
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Target: 5'- cGGAcgugaaAGCAgGCAGGCCAUggggCGUGCAu -3' miRNA: 3'- -UCUaug---UCGUgUGUCUGGUG----GCAUGU- -5' |
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| 1007 | 5' | -51.2 | NC_000924.1 | + | 51582 | 0.67 | 0.87774 |
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Target: 5'- ---cACAGCAgGCAGAaguaCGCCG-ACAg -3' miRNA: 3'- ucuaUGUCGUgUGUCUg---GUGGCaUGU- -5' |
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| 1007 | 5' | -51.2 | NC_000924.1 | + | 16151 | 0.67 | 0.87774 |
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Target: 5'- aAGAacACGGuCACGCGGAUagCAUCGUGCGg -3' miRNA: 3'- -UCUa-UGUC-GUGUGUCUG--GUGGCAUGU- -5' |
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| 1007 | 5' | -51.2 | NC_000924.1 | + | 7892 | 0.72 | 0.633729 |
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Target: 5'- cGGAaggGCAGCcaGCaACAGGCCACCcUGCAa -3' miRNA: 3'- -UCUa--UGUCG--UG-UGUCUGGUGGcAUGU- -5' |
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| 1007 | 5' | -51.2 | NC_000924.1 | + | 36094 | 0.74 | 0.500597 |
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Target: 5'- cGGuUGCAGUACcCAGACCACCcGUugAa -3' miRNA: 3'- -UCuAUGUCGUGuGUCUGGUGG-CAugU- -5' |
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| 1007 | 5' | -51.2 | NC_000924.1 | + | 25356 | 1.1 | 0.002489 |
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Target: 5'- gAGAUACAGCACACAGACCACCGUACAg -3' miRNA: 3'- -UCUAUGUCGUGUGUCUGGUGGCAUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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