Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1009 | 3' | -52.8 | NC_000924.1 | + | 53845 | 0.67 | 0.784997 |
Target: 5'- uGGCGGagaaaCAGCCGaAUAAAUuCGUGGCGGc -3' miRNA: 3'- -UUGCC-----GUCGGC-UAUUUG-GUAUCGCCu -5' |
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1009 | 3' | -52.8 | NC_000924.1 | + | 32092 | 0.67 | 0.753912 |
Target: 5'- aGGCGGUAGCUGuggcAUCcgGGCGGGu -3' miRNA: 3'- -UUGCCGUCGGCuauuUGGuaUCGCCU- -5' |
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1009 | 3' | -52.8 | NC_000924.1 | + | 57536 | 0.69 | 0.643685 |
Target: 5'- aAAUGGCAgcguauuuGCUGAaAAAgCAUGGCGGAu -3' miRNA: 3'- -UUGCCGU--------CGGCUaUUUgGUAUCGCCU- -5' |
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1009 | 3' | -52.8 | NC_000924.1 | + | 54981 | 0.71 | 0.565114 |
Target: 5'- gGACGGCGGuUCGGggauGACCAacGCGGAa -3' miRNA: 3'- -UUGCCGUC-GGCUau--UUGGUauCGCCU- -5' |
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1009 | 3' | -52.8 | NC_000924.1 | + | 16329 | 0.73 | 0.42823 |
Target: 5'- uGCGGCGGCUGAaaaaugGAACCGgagAGCaGGAg -3' miRNA: 3'- uUGCCGUCGGCUa-----UUUGGUa--UCG-CCU- -5' |
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1009 | 3' | -52.8 | NC_000924.1 | + | 55739 | 0.76 | 0.289885 |
Target: 5'- aGACGGCGG-UGAUGAACCugaaGGCGGAg -3' miRNA: 3'- -UUGCCGUCgGCUAUUUGGua--UCGCCU- -5' |
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1009 | 3' | -52.8 | NC_000924.1 | + | 26124 | 1.07 | 0.002243 |
Target: 5'- gAACGGCAGCCGAUAAACCAUAGCGGAg -3' miRNA: 3'- -UUGCCGUCGGCUAUUUGGUAUCGCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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