Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1012 | 3' | -54.5 | NC_000924.1 | + | 13382 | 0.66 | 0.764647 |
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Target: 5'- -cAUCuuUGCCagaCCGCGcugGGUAAGCGUu -3' miRNA: 3'- aaUAGcgACGGg--GGCGC---UUAUUCGCG- -5' |
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| 1012 | 3' | -54.5 | NC_000924.1 | + | 11743 | 0.67 | 0.678806 |
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Target: 5'- -gAUUGCUGCUUCUuCGGAUGAGUGa -3' miRNA: 3'- aaUAGCGACGGGGGcGCUUAUUCGCg -5' |
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| 1012 | 3' | -54.5 | NC_000924.1 | + | 37342 | 0.7 | 0.535336 |
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Target: 5'- --uUCGCUgGCCCCUGCagGAAUu-GUGCg -3' miRNA: 3'- aauAGCGA-CGGGGGCG--CUUAuuCGCG- -5' |
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| 1012 | 3' | -54.5 | NC_000924.1 | + | 27724 | 0.71 | 0.482556 |
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Target: 5'- aUcgCGCUGCgCUuuGUaGGUGAGCGCg -3' miRNA: 3'- aAuaGCGACG-GGggCGcUUAUUCGCG- -5' |
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| 1012 | 3' | -54.5 | NC_000924.1 | + | 33305 | 0.73 | 0.358939 |
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Target: 5'- ---aCGCUGCCCCUGUGucaGGGCGa -3' miRNA: 3'- aauaGCGACGGGGGCGCuuaUUCGCg -5' |
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| 1012 | 3' | -54.5 | NC_000924.1 | + | 24447 | 0.8 | 0.120704 |
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Target: 5'- cUGUCGaugaUGCCCCCGCGuuu-AGCGCc -3' miRNA: 3'- aAUAGCg---ACGGGGGCGCuuauUCGCG- -5' |
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| 1012 | 3' | -54.5 | NC_000924.1 | + | 33541 | 1.1 | 0.000986 |
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Target: 5'- cUUAUCGCUGCCCCCGCGAAUAAGCGCa -3' miRNA: 3'- -AAUAGCGACGGGGGCGCUUAUUCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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