Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1013 | 3' | -48.4 | NC_000924.1 | + | 29785 | 0.66 | 0.982256 |
Target: 5'- uGCUCcggaacaACUGGCgcugCGCCGGucgcauucugaAGGUGACCa -3' miRNA: 3'- -UGAG-------UGACUGaa--GUGGCU-----------UUCGUUGG- -5' |
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1013 | 3' | -48.4 | NC_000924.1 | + | 49519 | 0.66 | 0.977622 |
Target: 5'- -aUCACUGACagccUUACCGGuuGCcuucaGACCu -3' miRNA: 3'- ugAGUGACUGa---AGUGGCUuuCG-----UUGG- -5' |
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1013 | 3' | -48.4 | NC_000924.1 | + | 19950 | 0.66 | 0.974851 |
Target: 5'- -aUCGCUgGACUUCGaaGAAugcagGGCGAUCa -3' miRNA: 3'- ugAGUGA-CUGAAGUggCUU-----UCGUUGG- -5' |
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1013 | 3' | -48.4 | NC_000924.1 | + | 49405 | 0.67 | 0.96505 |
Target: 5'- -gUUACUGAUauugUCGguaaagguCUGAAGGCAACCg -3' miRNA: 3'- ugAGUGACUGa---AGU--------GGCUUUCGUUGG- -5' |
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1013 | 3' | -48.4 | NC_000924.1 | + | 24495 | 0.67 | 0.961257 |
Target: 5'- cGC-CugUGGCUUCugcACCGgAAGGCGAUg -3' miRNA: 3'- -UGaGugACUGAAG---UGGC-UUUCGUUGg -5' |
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1013 | 3' | -48.4 | NC_000924.1 | + | 31206 | 0.68 | 0.938039 |
Target: 5'- aGCUCAUgaGCUgaUCAUC-AAAGCAGCCu -3' miRNA: 3'- -UGAGUGacUGA--AGUGGcUUUCGUUGG- -5' |
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1013 | 3' | -48.4 | NC_000924.1 | + | 46783 | 0.68 | 0.938039 |
Target: 5'- cCUU-CUGACcagaACCGAGAGCAGCa -3' miRNA: 3'- uGAGuGACUGaag-UGGCUUUCGUUGg -5' |
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1013 | 3' | -48.4 | NC_000924.1 | + | 16938 | 0.69 | 0.926686 |
Target: 5'- cGCUCACUGGCUa-ACUGAGGuCGACg -3' miRNA: 3'- -UGAGUGACUGAagUGGCUUUcGUUGg -5' |
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1013 | 3' | -48.4 | NC_000924.1 | + | 18987 | 0.69 | 0.926686 |
Target: 5'- gAC-CGCUGGCgagaACuCGAAggGGCAACCg -3' miRNA: 3'- -UGaGUGACUGaag-UG-GCUU--UCGUUGG- -5' |
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1013 | 3' | -48.4 | NC_000924.1 | + | 49849 | 0.7 | 0.885467 |
Target: 5'- uACUCugaGCUGGCgcagUCACCGAAauuucAGCAGa- -3' miRNA: 3'- -UGAG---UGACUGa---AGUGGCUU-----UCGUUgg -5' |
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1013 | 3' | -48.4 | NC_000924.1 | + | 2057 | 0.7 | 0.885467 |
Target: 5'- uGCUCACUGugUggcggaaaUCAUgGcAAAGCcGCCa -3' miRNA: 3'- -UGAGUGACugA--------AGUGgC-UUUCGuUGG- -5' |
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1013 | 3' | -48.4 | NC_000924.1 | + | 37998 | 0.71 | 0.823832 |
Target: 5'- gACggCGCUGACaguUUCACCGGAugcgcucAGCGGCa -3' miRNA: 3'- -UGa-GUGACUG---AAGUGGCUU-------UCGUUGg -5' |
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1013 | 3' | -48.4 | NC_000924.1 | + | 38527 | 0.73 | 0.753406 |
Target: 5'- cGCUgCugUGACUgCACgGGucGCAGCCu -3' miRNA: 3'- -UGA-GugACUGAaGUGgCUuuCGUUGG- -5' |
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1013 | 3' | -48.4 | NC_000924.1 | + | 25446 | 0.75 | 0.664039 |
Target: 5'- gGCUgACUGACUggCGCUGAuuuucucAGCGGCCc -3' miRNA: 3'- -UGAgUGACUGAa-GUGGCUu------UCGUUGG- -5' |
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1013 | 3' | -48.4 | NC_000924.1 | + | 34239 | 0.76 | 0.5612 |
Target: 5'- cGCUCACUGAacgUUUCGCUG-AAGUGGCCc -3' miRNA: 3'- -UGAGUGACU---GAAGUGGCuUUCGUUGG- -5' |
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1013 | 3' | -48.4 | NC_000924.1 | + | 34366 | 1.13 | 0.003185 |
Target: 5'- gACUCACUGACUUCACCGAAAGCAACCu -3' miRNA: 3'- -UGAGUGACUGAAGUGGCUUUCGUUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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