Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1014 | 3' | -58.1 | NC_000924.1 | + | 35298 | 0.66 | 0.625103 |
Target: 5'- gUCCUGuACGuCACGCCGcgucaguggaAUGACUGGUa -3' miRNA: 3'- -AGGGC-UGCcGUGUGGU----------UGCUGACCGc -5' |
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1014 | 3' | -58.1 | NC_000924.1 | + | 5784 | 0.66 | 0.625103 |
Target: 5'- cCCUGAaaGCACA---GCGGCUGGCu -3' miRNA: 3'- aGGGCUgcCGUGUgguUGCUGACCGc -5' |
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1014 | 3' | -58.1 | NC_000924.1 | + | 43526 | 0.66 | 0.625103 |
Target: 5'- uUUCCGuucugaauaACGGCACuCCGGauaACUGGCGa -3' miRNA: 3'- -AGGGC---------UGCCGUGuGGUUgc-UGACCGC- -5' |
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1014 | 3' | -58.1 | NC_000924.1 | + | 26225 | 0.66 | 0.624039 |
Target: 5'- aCCCGcaccaucACGGUAUGCUGugGugUGGUu -3' miRNA: 3'- aGGGC-------UGCCGUGUGGUugCugACCGc -5' |
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1014 | 3' | -58.1 | NC_000924.1 | + | 30221 | 0.66 | 0.603845 |
Target: 5'- -gCUGACGGCACAggauuauaaauuCCAuuuuuACGcCUGGUGg -3' miRNA: 3'- agGGCUGCCGUGU------------GGU-----UGCuGACCGC- -5' |
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1014 | 3' | -58.1 | NC_000924.1 | + | 26506 | 0.66 | 0.603845 |
Target: 5'- aUCCCGuCGugaccaggagaGCGCGCUGGCGuGCUGGgGa -3' miRNA: 3'- -AGGGCuGC-----------CGUGUGGUUGC-UGACCgC- -5' |
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1014 | 3' | -58.1 | NC_000924.1 | + | 15080 | 0.66 | 0.597483 |
Target: 5'- uUCCUgucaugGGCGGCAgACCucuaaaucguguucaGGCG-CUGGCGa -3' miRNA: 3'- -AGGG------CUGCCGUgUGG---------------UUGCuGACCGC- -5' |
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1014 | 3' | -58.1 | NC_000924.1 | + | 6214 | 0.67 | 0.572149 |
Target: 5'- cUCCCGAUGGUuuauGCAgUGACGGCaacGGCc -3' miRNA: 3'- -AGGGCUGCCG----UGUgGUUGCUGa--CCGc -5' |
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1014 | 3' | -58.1 | NC_000924.1 | + | 3711 | 0.67 | 0.561665 |
Target: 5'- -gCUGGCugugcaaaagaGGCGCGCaagGAUGGCUGGCGa -3' miRNA: 3'- agGGCUG-----------CCGUGUGg--UUGCUGACCGC- -5' |
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1014 | 3' | -58.1 | NC_000924.1 | + | 23982 | 0.67 | 0.540862 |
Target: 5'- aCCUGGCggauauGGCAgGCCAguGCGucgguggcucuGCUGGCGg -3' miRNA: 3'- aGGGCUG------CCGUgUGGU--UGC-----------UGACCGC- -5' |
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1014 | 3' | -58.1 | NC_000924.1 | + | 31828 | 0.68 | 0.487142 |
Target: 5'- uUCCCGGgaaUGGCucagguuaucgauuAUGCCAuugAUGACUGGCGu -3' miRNA: 3'- -AGGGCU---GCCG--------------UGUGGU---UGCUGACCGC- -5' |
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1014 | 3' | -58.1 | NC_000924.1 | + | 23754 | 0.69 | 0.460755 |
Target: 5'- -gCCGAUGGCACAUaCAgugacGCGAgUGGUGc -3' miRNA: 3'- agGGCUGCCGUGUG-GU-----UGCUgACCGC- -5' |
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1014 | 3' | -58.1 | NC_000924.1 | + | 27580 | 0.75 | 0.19084 |
Target: 5'- gUCCUGGCGGUugGCUAugGugcgcugugGCUGGCa -3' miRNA: 3'- -AGGGCUGCCGugUGGUugC---------UGACCGc -5' |
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1014 | 3' | -58.1 | NC_000924.1 | + | 36910 | 1.09 | 0.000632 |
Target: 5'- cUCCCGACGGCACACCAACGACUGGCGg -3' miRNA: 3'- -AGGGCUGCCGUGUGGUUGCUGACCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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