Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10198 | 3' | -51.3 | NC_002657.1 | + | 7108 | 0.66 | 0.486768 |
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Target: 5'- gGCUU-AGACugGCCa-GACCCUGGa -3' miRNA: 3'- aCGGGuUCUGugUGGaaUUGGGAUCg -5' |
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| 10198 | 3' | -51.3 | NC_002657.1 | + | 6902 | 0.67 | 0.428124 |
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Target: 5'- aUGgCCAGGACugACCacccagAACCaauucagCUGGCg -3' miRNA: 3'- -ACgGGUUCUGugUGGaa----UUGG-------GAUCG- -5' |
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| 10198 | 3' | -51.3 | NC_002657.1 | + | 3524 | 0.67 | 0.418212 |
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Target: 5'- aGCCaCAGGACAUAUCUUccUCCUAa- -3' miRNA: 3'- aCGG-GUUCUGUGUGGAAuuGGGAUcg -5' |
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| 10198 | 3' | -51.3 | NC_002657.1 | + | 5108 | 0.68 | 0.354876 |
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Target: 5'- cGuCCCAAGAUuuccgaccaggaGCAUCUUGACUuuuguugCUAGCa -3' miRNA: 3'- aC-GGGUUCUG------------UGUGGAAUUGG-------GAUCG- -5' |
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| 10198 | 3' | -51.3 | NC_002657.1 | + | 4866 | 0.7 | 0.250255 |
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Target: 5'- cUGCCCAaaaugcgggcguuuuGGGC-CACCaaugaccugugguaUGACCCUAGCc -3' miRNA: 3'- -ACGGGU---------------UCUGuGUGGa-------------AUUGGGAUCG- -5' |
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| 10198 | 3' | -51.3 | NC_002657.1 | + | 240 | 1.14 | 0.000126 |
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Target: 5'- aUGCCCAAGACACACCUUAACCCUAGCg -3' miRNA: 3'- -ACGGGUUCUGUGUGGAAUUGGGAUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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