Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1020 | 5' | -56.3 | NC_000924.1 | + | 16402 | 0.66 | 0.684384 |
Target: 5'- uUCAGCCGccgCAGCaacuGAu-GGUGCCCAUg -3' miRNA: 3'- -GGUCGGUa--GUCGg---CUuuCCGUGGGUG- -5' |
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1020 | 5' | -56.3 | NC_000924.1 | + | 48349 | 0.66 | 0.673555 |
Target: 5'- aCCAGCg--CAGCagGAAGGGUACgUACu -3' miRNA: 3'- -GGUCGguaGUCGg-CUUUCCGUGgGUG- -5' |
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1020 | 5' | -56.3 | NC_000924.1 | + | 17582 | 0.66 | 0.65179 |
Target: 5'- -aGGCCAaaGGCgGuAAGGCACgCGCg -3' miRNA: 3'- ggUCGGUagUCGgCuUUCCGUGgGUG- -5' |
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1020 | 5' | -56.3 | NC_000924.1 | + | 31861 | 0.66 | 0.6507 |
Target: 5'- uCCAGCggcgacgCAUCAGCC-AucGGCAgUCACu -3' miRNA: 3'- -GGUCG-------GUAGUCGGcUuuCCGUgGGUG- -5' |
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1020 | 5' | -56.3 | NC_000924.1 | + | 23349 | 0.66 | 0.640877 |
Target: 5'- aCCAGCUgucgaaAUCAGCgCacuaccauAAGGCugCCACc -3' miRNA: 3'- -GGUCGG------UAGUCG-Gcu------UUCCGugGGUG- -5' |
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1020 | 5' | -56.3 | NC_000924.1 | + | 19146 | 0.67 | 0.615761 |
Target: 5'- uUCAGCCuucuggucuucaagGUCGGCCGcAAGGCGCa--- -3' miRNA: 3'- -GGUCGG--------------UAGUCGGCuUUCCGUGggug -5' |
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1020 | 5' | -56.3 | NC_000924.1 | + | 26854 | 0.67 | 0.608127 |
Target: 5'- aCAGgaAUcCAGCCcacaGAAAGGCACCgACa -3' miRNA: 3'- gGUCggUA-GUCGG----CUUUCCGUGGgUG- -5' |
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1020 | 5' | -56.3 | NC_000924.1 | + | 50484 | 0.67 | 0.597238 |
Target: 5'- gCAGCuCAUC-GCCGGGAaGCugCCAg -3' miRNA: 3'- gGUCG-GUAGuCGGCUUUcCGugGGUg -5' |
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1020 | 5' | -56.3 | NC_000924.1 | + | 49600 | 0.67 | 0.586379 |
Target: 5'- uUCGGUCAUCGGCguauucagcgcaCGAAcuGCACCCGg -3' miRNA: 3'- -GGUCGGUAGUCG------------GCUUucCGUGGGUg -5' |
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1020 | 5' | -56.3 | NC_000924.1 | + | 45780 | 0.71 | 0.371791 |
Target: 5'- cCCcGUCAacugguugcugagguUCAGCCGAuauGGCACCCGu -3' miRNA: 3'- -GGuCGGU---------------AGUCGGCUuu-CCGUGGGUg -5' |
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1020 | 5' | -56.3 | NC_000924.1 | + | 39594 | 0.73 | 0.311462 |
Target: 5'- aCCGGaCC-UCaggGGCCGAAGGGCgACCCGg -3' miRNA: 3'- -GGUC-GGuAG---UCGGCUUUCCG-UGGGUg -5' |
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1020 | 5' | -56.3 | NC_000924.1 | + | 25371 | 0.74 | 0.242186 |
Target: 5'- gCCAGUCAgUCAGCCGGAAGcaaACCCGa -3' miRNA: 3'- -GGUCGGU-AGUCGGCUUUCcg-UGGGUg -5' |
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1020 | 5' | -56.3 | NC_000924.1 | + | 22865 | 0.76 | 0.186231 |
Target: 5'- aCCAgGCCGaacUCA-CCGGGAGGCACCCGg -3' miRNA: 3'- -GGU-CGGU---AGUcGGCUUUCCGUGGGUg -5' |
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1020 | 5' | -56.3 | NC_000924.1 | + | 45551 | 1.12 | 0.000543 |
Target: 5'- uCCAGCCAUCAGCCGAAAGGCACCCACg -3' miRNA: 3'- -GGUCGGUAGUCGGCUUUCCGUGGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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