Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
10201 | 3' | -48.9 | NC_002665.1 | + | 14563 | 0.66 | 0.994073 |
Target: 5'- gGGAAGAcCCAGGACGgugcgAGuGuCUGuGUCa -3' miRNA: 3'- -UCUUCUaGGUCCUGU-----UC-CuGACuUAG- -5' |
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10201 | 3' | -48.9 | NC_002665.1 | + | 13930 | 0.66 | 0.993098 |
Target: 5'- -----cUCCGGG-UAGGGGCUGAAUg -3' miRNA: 3'- ucuucuAGGUCCuGUUCCUGACUUAg -5' |
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10201 | 3' | -48.9 | NC_002665.1 | + | 4560 | 0.67 | 0.990765 |
Target: 5'- --uGGAUCUGGGugGGGGGgUGGAg- -3' miRNA: 3'- ucuUCUAGGUCCugUUCCUgACUUag -5' |
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10201 | 3' | -48.9 | NC_002665.1 | + | 89189 | 0.67 | 0.990765 |
Target: 5'- gGGggGGUuuGGGACuggguGGGGGCcacgGggUCu -3' miRNA: 3'- -UCuuCUAggUCCUG-----UUCCUGa---CuuAG- -5' |
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10201 | 3' | -48.9 | NC_002665.1 | + | 108583 | 0.67 | 0.987854 |
Target: 5'- -uGGGGUggGGGACGAGGGCUgugGAAUCc -3' miRNA: 3'- ucUUCUAggUCCUGUUCCUGA---CUUAG- -5' |
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10201 | 3' | -48.9 | NC_002665.1 | + | 77617 | 0.67 | 0.987854 |
Target: 5'- aAGAGGggCCAGGGggaAAGGGCcaUGggUa -3' miRNA: 3'- -UCUUCuaGGUCCUg--UUCCUG--ACuuAg -5' |
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10201 | 3' | -48.9 | NC_002665.1 | + | 16492 | 0.67 | 0.987854 |
Target: 5'- uGAGGcAUCUGGGucACAAGGuACUGggUa -3' miRNA: 3'- uCUUC-UAGGUCC--UGUUCC-UGACuuAg -5' |
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10201 | 3' | -48.9 | NC_002665.1 | + | 71880 | 0.67 | 0.984278 |
Target: 5'- aGGGAGAUaCAGGAgaCGGGGACU--AUCg -3' miRNA: 3'- -UCUUCUAgGUCCU--GUUCCUGAcuUAG- -5' |
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10201 | 3' | -48.9 | NC_002665.1 | + | 15601 | 0.68 | 0.974802 |
Target: 5'- cAGGAGAggccgguagagCCAGGAUAGGGAgcuaCUGuGUCc -3' miRNA: 3'- -UCUUCUa----------GGUCCUGUUCCU----GACuUAG- -5' |
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10201 | 3' | -48.9 | NC_002665.1 | + | 24336 | 0.71 | 0.922985 |
Target: 5'- uGGAGGAUaugagggGGGAgGAGGGCUGGAUa -3' miRNA: 3'- -UCUUCUAgg-----UCCUgUUCCUGACUUAg -5' |
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10201 | 3' | -48.9 | NC_002665.1 | + | 70567 | 0.74 | 0.804312 |
Target: 5'- --cGGGUCCaAGGAUAuggagaacauccAGGACUGAAUCc -3' miRNA: 3'- ucuUCUAGG-UCCUGU------------UCCUGACUUAG- -5' |
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10201 | 3' | -48.9 | NC_002665.1 | + | 15779 | 1.12 | 0.006157 |
Target: 5'- cAGAAGAUCCAGGACAAGGACUGAAUCa -3' miRNA: 3'- -UCUUCUAGGUCCUGUUCCUGACUUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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