miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
10201 5' -55.3 NC_002665.1 + 108224 0.67 0.830169
Target:  5'- --uGCUCCgUGgaugCCUGGCUCUguuccuaGCCa- -3'
miRNA:   3'- cauCGAGGgAUa---GGACCGAGA-------UGGcc -5'
10201 5' -55.3 NC_002665.1 + 66777 0.68 0.785799
Target:  5'- -cGGC-CCCUAUCaUGGCuucccUCUgaGCCGGg -3'
miRNA:   3'- caUCGaGGGAUAGgACCG-----AGA--UGGCC- -5'
10201 5' -55.3 NC_002665.1 + 15965 0.68 0.75683
Target:  5'- uGUGGCgCCCUGauagccaCCUGGCUUUugggccCCGGg -3'
miRNA:   3'- -CAUCGaGGGAUa------GGACCGAGAu-----GGCC- -5'
10201 5' -55.3 NC_002665.1 + 59079 0.69 0.726811
Target:  5'- -aAGC-CCCUcgCCUGGCauuuuauaaagUCUGCCa- -3'
miRNA:   3'- caUCGaGGGAuaGGACCG-----------AGAUGGcc -5'
10201 5' -55.3 NC_002665.1 + 2069 0.69 0.696013
Target:  5'- uGUGGCUCCCauUGUUUUGGUggCUAUCGa -3'
miRNA:   3'- -CAUCGAGGG--AUAGGACCGa-GAUGGCc -5'
10201 5' -55.3 NC_002665.1 + 15744 1.1 0.002005
Target:  5'- aGUAGCUCCCUAUCCUGGCUCUACCGGc -3'
miRNA:   3'- -CAUCGAGGGAUAGGACCGAGAUGGCC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.