Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
10201 | 5' | -55.3 | NC_002665.1 | + | 108224 | 0.67 | 0.830169 |
Target: 5'- --uGCUCCgUGgaugCCUGGCUCUguuccuaGCCa- -3' miRNA: 3'- cauCGAGGgAUa---GGACCGAGA-------UGGcc -5' |
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10201 | 5' | -55.3 | NC_002665.1 | + | 66777 | 0.68 | 0.785799 |
Target: 5'- -cGGC-CCCUAUCaUGGCuucccUCUgaGCCGGg -3' miRNA: 3'- caUCGaGGGAUAGgACCG-----AGA--UGGCC- -5' |
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10201 | 5' | -55.3 | NC_002665.1 | + | 15965 | 0.68 | 0.75683 |
Target: 5'- uGUGGCgCCCUGauagccaCCUGGCUUUugggccCCGGg -3' miRNA: 3'- -CAUCGaGGGAUa------GGACCGAGAu-----GGCC- -5' |
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10201 | 5' | -55.3 | NC_002665.1 | + | 59079 | 0.69 | 0.726811 |
Target: 5'- -aAGC-CCCUcgCCUGGCauuuuauaaagUCUGCCa- -3' miRNA: 3'- caUCGaGGGAuaGGACCG-----------AGAUGGcc -5' |
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10201 | 5' | -55.3 | NC_002665.1 | + | 2069 | 0.69 | 0.696013 |
Target: 5'- uGUGGCUCCCauUGUUUUGGUggCUAUCGa -3' miRNA: 3'- -CAUCGAGGG--AUAGGACCGa-GAUGGCc -5' |
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10201 | 5' | -55.3 | NC_002665.1 | + | 15744 | 1.1 | 0.002005 |
Target: 5'- aGUAGCUCCCUAUCCUGGCUCUACCGGc -3' miRNA: 3'- -CAUCGAGGGAUAGGACCGAGAUGGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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