Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10202 | 3' | -50.4 | NC_002665.1 | + | 24770 | 0.67 | 0.963434 |
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Target: 5'- aUCUGAUaucCUGACUGCCaaGGCCAu -3' miRNA: 3'- -GGACUAaccGAUUGACGGaaCUGGUu -5' |
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| 10202 | 3' | -50.4 | NC_002665.1 | + | 105964 | 0.68 | 0.941466 |
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Target: 5'- uCCUGucuagaaccauauGUUGGCUGauaaACUGUUUUGGCCu- -3' miRNA: 3'- -GGAC-------------UAACCGAU----UGACGGAACUGGuu -5' |
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| 10202 | 3' | -50.4 | NC_002665.1 | + | 12015 | 0.69 | 0.913643 |
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Target: 5'- cCCU-AUUGGUaacAGCUGCCUggGACCAc -3' miRNA: 3'- -GGAcUAACCGa--UUGACGGAa-CUGGUu -5' |
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| 10202 | 3' | -50.4 | NC_002665.1 | + | 97477 | 0.71 | 0.845639 |
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Target: 5'- cCCUGAUUGGUgGAUUGCUgaaaGCCAAu -3' miRNA: 3'- -GGACUAACCGaUUGACGGaac-UGGUU- -5' |
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| 10202 | 3' | -50.4 | NC_002665.1 | + | 21232 | 0.79 | 0.429415 |
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Target: 5'- uCCaUGAUUGGCUGACUGCCaugagGACgGAc -3' miRNA: 3'- -GG-ACUAACCGAUUGACGGaa---CUGgUU- -5' |
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| 10202 | 3' | -50.4 | NC_002665.1 | + | 21403 | 1.09 | 0.005616 |
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Target: 5'- aCCUGAUUGGCUAACUGCCUUGACCAAu -3' miRNA: 3'- -GGACUAACCGAUUGACGGAACUGGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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