Results 1 - 18 of 18 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
10203 | 3' | -64 | NC_002665.1 | + | 96912 | 0.66 | 0.466079 |
Target: 5'- -cGCCUCCCUucCCCUCcCCGGGGUc -3' miRNA: 3'- guCGGGGGGGuuGGGGGuGGUCUCGc -5' |
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10203 | 3' | -64 | NC_002665.1 | + | 23976 | 0.66 | 0.448115 |
Target: 5'- cCAGCCCCcaaucuccCCCaAACCCCCAagaccccaacCCAGuGUc -3' miRNA: 3'- -GUCGGGG--------GGG-UUGGGGGU----------GGUCuCGc -5' |
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10203 | 3' | -64 | NC_002665.1 | + | 90138 | 0.66 | 0.448115 |
Target: 5'- cCAuCCCCCCCAAaCCCCAaacCCucGAGCc -3' miRNA: 3'- -GUcGGGGGGGUUgGGGGU---GGu-CUCGc -5' |
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10203 | 3' | -64 | NC_002665.1 | + | 25060 | 0.66 | 0.439277 |
Target: 5'- aAGCCUCCCgcaCAAUCCCUAcuCCAGAuGCu -3' miRNA: 3'- gUCGGGGGG---GUUGGGGGU--GGUCU-CGc -5' |
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10203 | 3' | -64 | NC_002665.1 | + | 72173 | 0.67 | 0.421906 |
Target: 5'- aCAGaauaCCUCCCCGGCCCgCCACUGGAc-- -3' miRNA: 3'- -GUC----GGGGGGGUUGGG-GGUGGUCUcgc -5' |
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10203 | 3' | -64 | NC_002665.1 | + | 141 | 0.67 | 0.413377 |
Target: 5'- aCAGCCCUCCaCGGCCCuuCCcCCAcAGUGa -3' miRNA: 3'- -GUCGGGGGG-GUUGGG--GGuGGUcUCGC- -5' |
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10203 | 3' | -64 | NC_002665.1 | + | 96320 | 0.67 | 0.396642 |
Target: 5'- cUAGCCUCCCUu-CCCCUuccCCGGGGUc -3' miRNA: 3'- -GUCGGGGGGGuuGGGGGu--GGUCUCGc -5' |
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10203 | 3' | -64 | NC_002665.1 | + | 101504 | 0.67 | 0.396642 |
Target: 5'- uGGCUCCCaaAGCUCCUauauggguGCCAGAGCc -3' miRNA: 3'- gUCGGGGGggUUGGGGG--------UGGUCUCGc -5' |
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10203 | 3' | -64 | NC_002665.1 | + | 89873 | 0.67 | 0.380349 |
Target: 5'- aAGCCaCuCCCCAACcaagaagacauCCCCACCAcAGCc -3' miRNA: 3'- gUCGG-G-GGGGUUG-----------GGGGUGGUcUCGc -5' |
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10203 | 3' | -64 | NC_002665.1 | + | 100253 | 0.67 | 0.378745 |
Target: 5'- gCGGCacagaCCCCCGACagaaucgCCCCAUCAuuccccuGAGCGa -3' miRNA: 3'- -GUCGg----GGGGGUUG-------GGGGUGGU-------CUCGC- -5' |
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10203 | 3' | -64 | NC_002665.1 | + | 105000 | 0.67 | 0.375549 |
Target: 5'- gCAGaCCCCCCUcACCCcaugaauaugaugaaCCACCAGAuGaCGg -3' miRNA: 3'- -GUC-GGGGGGGuUGGG---------------GGUGGUCU-C-GC- -5' |
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10203 | 3' | -64 | NC_002665.1 | + | 68313 | 0.68 | 0.334224 |
Target: 5'- aAGaCCCaaaCCCCAuagACCCCCGCCGG-GUa -3' miRNA: 3'- gUC-GGG---GGGGU---UGGGGGUGGUCuCGc -5' |
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10203 | 3' | -64 | NC_002665.1 | + | 4425 | 0.68 | 0.334224 |
Target: 5'- uCAGCCUCCaCC--CCCCCACCcAGAuccauuGCGa -3' miRNA: 3'- -GUCGGGGG-GGuuGGGGGUGG-UCU------CGC- -5' |
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10203 | 3' | -64 | NC_002665.1 | + | 80401 | 0.69 | 0.319786 |
Target: 5'- aCAGCuCCCCCCcACCCCaacaGCCAcaauuAGCu -3' miRNA: 3'- -GUCG-GGGGGGuUGGGGg---UGGUc----UCGc -5' |
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10203 | 3' | -64 | NC_002665.1 | + | 43646 | 0.7 | 0.260643 |
Target: 5'- gCAGCaCCCUCCAAUCCCUGCaCGG-GUGa -3' miRNA: 3'- -GUCG-GGGGGGUUGGGGGUG-GUCuCGC- -5' |
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10203 | 3' | -64 | NC_002665.1 | + | 17703 | 0.71 | 0.215837 |
Target: 5'- aCAGCgCCCCCAgaaaccauACCCCC-CCAGcacAGCc -3' miRNA: 3'- -GUCGgGGGGGU--------UGGGGGuGGUC---UCGc -5' |
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10203 | 3' | -64 | NC_002665.1 | + | 68482 | 0.73 | 0.173481 |
Target: 5'- -uGCCCCCCCAACgCCCAau-GGGCc -3' miRNA: 3'- guCGGGGGGGUUGgGGGUgguCUCGc -5' |
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10203 | 3' | -64 | NC_002665.1 | + | 23919 | 1.07 | 0.000519 |
Target: 5'- cCAGCCCCCCCAACCCCCACCAGAGCGc -3' miRNA: 3'- -GUCGGGGGGGUUGGGGGUGGUCUCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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