Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
10203 | 5' | -48.1 | NC_002665.1 | + | 9977 | 0.66 | 0.997552 |
Target: 5'- -cCACCCcGUGGccccaGAGCcAGUUcAAGGa -3' miRNA: 3'- gaGUGGGuCACC-----UUCGaUCAAaUUCC- -5' |
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10203 | 5' | -48.1 | NC_002665.1 | + | 44316 | 0.66 | 0.997552 |
Target: 5'- uUUACCUGGUGGAuuuGCUA-UUaAAGGg -3' miRNA: 3'- gAGUGGGUCACCUu--CGAUcAAaUUCC- -5' |
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10203 | 5' | -48.1 | NC_002665.1 | + | 23784 | 0.67 | 0.995159 |
Target: 5'- -gCGCuCUGGUGGggGUUGGg--GGGGc -3' miRNA: 3'- gaGUG-GGUCACCuuCGAUCaaaUUCC- -5' |
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10203 | 5' | -48.1 | NC_002665.1 | + | 96215 | 0.67 | 0.992329 |
Target: 5'- --aACCCGGggaaGGAGGCUAGggaacccGGGGa -3' miRNA: 3'- gagUGGGUCa---CCUUCGAUCaaa----UUCC- -5' |
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10203 | 5' | -48.1 | NC_002665.1 | + | 96259 | 0.67 | 0.992329 |
Target: 5'- --aACCCGGggaaGGAGGCUAGggaacccGGGGa -3' miRNA: 3'- gagUGGGUCa---CCUUCGAUCaaa----UUCC- -5' |
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10203 | 5' | -48.1 | NC_002665.1 | + | 96809 | 0.67 | 0.992329 |
Target: 5'- --aACCCGGggaaGGAGGCUAGggaacccGGGGa -3' miRNA: 3'- gagUGGGUCa---CCUUCGAUCaaa----UUCC- -5' |
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10203 | 5' | -48.1 | NC_002665.1 | + | 96325 | 0.68 | 0.990622 |
Target: 5'- --aACCCGGggaaGGAGGCUAGggaacccggggAAGGg -3' miRNA: 3'- gagUGGGUCa---CCUUCGAUCaaa--------UUCC- -5' |
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10203 | 5' | -48.1 | NC_002665.1 | + | 96403 | 0.68 | 0.990622 |
Target: 5'- --aACCCGGggaaGGAGGCUAGggaacccggggAAGGg -3' miRNA: 3'- gagUGGGUCa---CCUUCGAUCaaa--------UUCC- -5' |
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10203 | 5' | -48.1 | NC_002665.1 | + | 96454 | 0.68 | 0.990622 |
Target: 5'- --aACCCGGggaaGGAGGCUAGggaacccggggAAGGg -3' miRNA: 3'- gagUGGGUCa---CCUUCGAUCaaa--------UUCC- -5' |
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10203 | 5' | -48.1 | NC_002665.1 | + | 96527 | 0.68 | 0.990622 |
Target: 5'- --aACCCGGggaaGGAGGCUAGggaacccggggAAGGg -3' miRNA: 3'- gagUGGGUCa---CCUUCGAUCaaa--------UUCC- -5' |
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10203 | 5' | -48.1 | NC_002665.1 | + | 96853 | 0.68 | 0.990622 |
Target: 5'- --aACCCGGggaaGGAGGCUAGggaacccggggAAGGg -3' miRNA: 3'- gagUGGGUCa---CCUUCGAUCaaa--------UUCC- -5' |
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10203 | 5' | -48.1 | NC_002665.1 | + | 98368 | 0.68 | 0.988004 |
Target: 5'- cCUCGCCCA-UGGAuugagcggGGCgcuuuucccuggGGUUUAGGGu -3' miRNA: 3'- -GAGUGGGUcACCU--------UCGa-----------UCAAAUUCC- -5' |
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10203 | 5' | -48.1 | NC_002665.1 | + | 23885 | 1.12 | 0.007974 |
Target: 5'- aCUCACCCAGUGGAAGCUAGUUUAAGGc -3' miRNA: 3'- -GAGUGGGUCACCUUCGAUCAAAUUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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